海带多糖对脂多糖致血管内皮细胞炎性反应的影响

The Effect of Laminaria Polysaecharide On Inflammatory Stress Induced By Lipopolysaccharide In Vascular Endothelial Cells

目的:探讨海带多糖(LP)对脂多糖(LPS)致血管内皮细胞(Vascular endothelial cells,VECs)炎性反应的调整作用。方法:将70只昆明小鼠随机分为7个组:LP高剂量组(LP-H)、LP中剂量组(LP-M)、LP低剂量组(LP-L),此三组分别于尾静脉注射LP 50、25、10 mg/kg和LPS 3 mg/kg;依诺肝素组(依诺肝素2 IU/kg+LPS3 mg/kg),模型组(LPS 3 mg/kg),LP对照组(LP 50 mg/kg),空白对照组(生理盐水)。检测小鼠肛温;ELISA法测定小鼠血清白细胞介素1β(IL-1β)、白细胞介素8(IL-8)、肿瘤坏死因子-α(TNF-α)、一氧化氮(NO)、前列环素(PGI2)含量;RT-PCR法测定主动脉内皮型一氧化氮合酶(e NOS)、诱导型一氧化氮合酶(i NOS)、环氧合酶-2(COX-2)m RNA的表达。结果:LP能够降低LPS炎症小鼠体温(P<0.05),降低血清IL-1β、IL-8、TNF-a、NO、PGI2水平(P<0.05-0.01);上调e NOS m RNA表达(P<0.01);下调i NOS m RNA、COX-2 m RNA表达(P<0.01)。结论:LP在LPS炎性反应中抑制炎症因子产生,调整内皮相关基因的表达。

Objective：To investigate the effect of Laminaria Polysaecharide（LP）on inflammatory stress induced by lipopolysaccharide（LPS）in vascular endothelial cells（VECs）. Methods：70 Kunming mice were divided into 7 groups randomly：high dose LP group（H-LP）,middle dose LP group（M-LP）,low dose LP group（L-LP）,those were treated with LP 50、25、10 mg/kg and LPS 3 mg/kg from tail vein;enoxaparin group（enoxaparin 2 IU/kg ＋ LPS 3 mg/kg）,model group（LPS 3 mg/kg）,LP control group（LP 50 mg/kg）,saline control group（physiological saline）. Rectal temperature in mice was measured. Serum level of IL-1β,IL-8,TNF-α,NO,6-keto-PGF1α were measured by ELISA;The expressions of i NOS m RNA,e NOS m RNA and COX-2 m RNA in aorta were detected by RT-PCR. Results：LP can decrease body temperature of LPS inflammatory mice;reduce the Serum level of IL-1β,IL-8,TNF-α,NO,6-ketoPGF1α（P0.05-0.01）;up-regulate the expression of e NOS m RNA（P0.01）,down-regulate the expression of i NOS m RNA and COX-2 m RNA（P0.01）. Conclusion：LP inhibits Inflammatory factors production,adjusts the expression of endothelium related genes in LPS induced inflammatory stress.