慢性淋巴细胞白血病患者RhD抗原减弱的原因分析1例

Analysis of potential causes of decreased RhD antigen activity in the patients with chronic lymphocytic leukemia: one case

目的分析1例慢性淋巴细胞白血病患者RhD抗原减弱的原因。方法应用血清学方法鉴定该例标本的ABO、Rh血型及红细胞血型抗体鉴定。PCR-SSP方法扩增RH基因。结果先证者为A型,RhD抗原盐水介质阴性,经间接2批抗人球蛋白方法(IAT)确认样本为RhD阳性,血清中检测抗-D。PCR-SSP检测RH基因为Dccee。结论慢性淋巴细胞白血病患者RhD抗原减弱,产生类抗-D,导致RhD抗原检测及配血困难。

Objective To investigate the possible causes of reduced expression of RhD antigen in the patient with chronic lymphocytic leukemia. Methods Tests of ABO blood groups,Rh blood groups and antibodies against red blood cells were performed using serological techniques. The RH genes were multiplied by polymerase chain reaction-sequence specific primer（ PCR-SSP）. Result The serological phenotype of the sample is identified as type A,which was positive to RhD phenotypes and was confirmed anti-D antibody positive in indirect coomb＇s test（ IAT）. RH genes were confirmed to be RH Dccee. Conclusion Patients with reduced expression of RhD antigen produce anti-D antibodies which is likely to be the cause of difficulties in RhD antigen detection and cross matching.