水蛭的酶解及其产物抗凝血活性评价

ENZYMATIC HYDROLYSIS OF LEECH AND THE ANTICOAGULANT ACTIVITY OF THE ENZYMATIC HYDROLYSATE

以水蛭为原料,研究不同酶解因素(酶种类、酶用量、酶解时间、pH值、酶解温度、底物浓度)对水蛭酶解产物抗凝血活性的影响规律,优化水蛭的酶解技术参数,并对酶解产物进行活性评价;研究结果表明,优化后的酶解技术条件为,适宜的水解酶为胰蛋白酶,酶用量7000 U/g,酶解时间7 h,酶解pH 8.3,酶解温度50℃,底物浓度14%(w/w)。在该条件下得到的酶解产物抗凝血酶活性最高,达到640 U/g。

Using leech as the raw material, effects of different factors （Enzyme type, enzyme concentration, enzymatic hydrolysis time, pH value, temperature, substrate concentration） on the anticoagulant activity of enzymatic hydrolysate were investigated, the enzymatic conditions were optimized, and the anticoagulant activity of enzymatic hydrolysate was evaluated. The results showed that the optimal conditions were as follows： the suitable hydrolas was trypsin with the dosage of 7000 U/g, hydrolysis time was 7 hours, enzymatic reaction pH was 8.3, temperature was 50℃ and substrate concentration was 14% （w/w）. Under above conditions, the anticoagulant activity of hydrolysate was the highest and reached 640 U/g.