联合检测survivin、VEGF-C和VEGFR-3蛋白表达水平在食管癌诊治中的意义

Combined detection of survivin,VEGF-C and VEGFR-3 expression and their clinical significance in diagnosis and treatment of esophageal cancer

目的探讨联合检测survivin、VEGF-C和VEGFR-3蛋白表达水平在食管癌诊治中的意义。方法采用免疫组化法检测141例食管癌手术切除标本中survivin、VEGF-C和VEGFR-3蛋白表达水平,分析三者与食管癌临床病理特征的关系以及三者的相互关系。结果 (1)在141例食管癌中105例survivin(+)。survivin表达与患者的病理T分期、淋巴结转移、神经束浸润和分化程度有相关性(P<0.05),与患者性别、年龄无相关性(P>0.05)。(2)141例中,110例VEGF-C(+);95例VEGFR-3(+)。VEGF-C和VEGFR-3表达与患者的病理T分期、淋巴结转移有相关性(P<0.05),与患者性别、年龄、神经束浸润、分化程度无相关性(P>0.05)。(3)VEGF-C与VEGFR-3在食管癌组织中的表达呈正相关(P<0.05)。(4)survivin与VEGF-C、VEGFR-3在食管癌组织中的表达呈正相关(P<0.05)。结论 survivin与食管癌侵袭及远处转移有关,肿瘤分期越晚、分化恶性度越高,survivin阳性率愈高。VEGF-C和VEGFR-3与食管癌淋巴结转移有关,VEGF-C和VEGFR-3高表达提示食管癌伴有淋巴结转移且预后不良。可以联合检测食管癌survivin、VEGF-C和VEGFR-3帮助食管癌的早期诊断和食管癌侵袭、淋巴结转移的判断,评估预后。

Objective To study the clinical significance of survivin and VEGF-C and VEGFR-3 protein expression level in the diagnosis and treatment of esophageal cancer. Methods The expression of survivin,VEGF-C and VEGFR-3 was detected in 141 cases of esophageal cancer with imunohistochemistry,and the relationship to clinical pathological features of esophageal cancer was analyzed. Results In 141 patients with esophageal cancer,the expression of survivin protein was positive in 105 cases. The expression of survivin had correlated with pathological T stages,lymph node metastasis,nerve invasion,and histopathological grade（ P 0. 05）,while no correlation with patients gender and age（ P 0. 05）. VEGF-C was positive in 110 cases; VEGFR-3 was positive in 95 cases; The expression of VEGF-C and VEGFR-3 had correlation with pathological T stages,lymph node metastasis（ P 0. 05）,while no correlation with patients gender,age,nerve invasion,and histopathological grade（ P 0. 05）. The expression of VEGF-C and VEGFR 3 had positively correlated（ P 0. 05）. The relationship between the three markers had positively correlated（ P 0. 05）. Conclusions The expression of survivin is related to the invasion and distant metastasis of esophageal carcinoma. In patients with more advanced tumor stage and higher malignancy,survivin positive rate is higher. VEGF-C and VEGFR-3 have correlation with lymph node metastasis. High expression of VEGF-C and VEGFR-3 suggests that patients would have lymph node metastasis and poor prognosis. Combined detection survivin,VEGF-C and VEGFR-3 helps make the diagnosis of early invasion and lymph node metastasis in esophageal carcinoma,and evaluate the prognosis of patients.