瑞安地区多重耐药结核分枝杆菌IS6110指纹图谱研究

Research on IS6110 fingerprinting of multi drug resistant Mycobacterium tuberculosis in Rui'an

目的了解温州瑞安市多重耐药结核分枝杆菌耐药IS6110指纹图谱特征。方法收集2013年1月-2017年3月本院结核患者的痰标本,进行结核培养,菌株鉴定,药物敏感实验,对利福平和异烟肼同时耐药的菌株进行RFLP分析。结果 516份培养阳性标本中468株(468/516,90.7%)为结核分枝杆菌,48株(48/516,9.3%)为非结核分枝杆菌。468株结核分枝杆菌对异烟肼、利福平、链霉素、乙胺丁醇的耐药率分别为12.8%、8.3%、7.7%、3.2%。根据耐药谱分析393株(393/468,84.0%)结核分枝杆菌对4种药物全部敏感,对利福平和异烟肼同时耐药的菌株有36株(36/468,7.7%)。36株多重耐药菌株RFLP分析主要分为2组。结论瑞安地区多重耐药结核分枝杆菌为散发存在,外来民工为高危人群,需要更好地对外来民工进行管理。

Objective To investigate the IS6110 fingerprint characteristics of multidrug-resistant Mycobacterium tuberculosis in Rui＇an,Wenzhou. Methods Sputum samples of tuberculosis patients in our hospital from January 2013 to March 2017 were collected for tuberculosis culture,bacterial identification and drug sensitivity test,and the strains simultaneously resistant to rifampin and isoniazid were analyzed by restriction fragment length polymorphism（ RFLP）. Results Among 516 cultured positive samples,468 strains（ 468/516,90. 7%） were Mycobacterium tuberculosis,48 strains（ 48/516,9. 3%） were non-Mycobacterium tuberculosis. The resistance rates of 468 Mycobacterium tuberculosis to isoniazid,rifampicin,streptomycin and ethambutol were 12. 8%,8. 3%,7. 7% and 3. 2%,respectively. According to the resistance spectrum analysis,393（ 393/468,84. 0%）strains of Mycobacterium tuberculosis were all sensitive to the four drugs,and 36（ 46/468,7. 7%） strains were resistant to rifampicin and isoniazid simultaneously. 36 strains of multi drug resistant strains of RFLP were divided into 2 groups. Conclusion The multi-drug resistant Mycobacterium tuberculosis in the Rui＇an area is exuded. Migrant workers are at high risk,and better management need to be strengthened on them.