甘草总黄酮提取部位抗惊厥作用的研究

Anticonvulsant Effects of the Total Flavonoids Extract Parts of Glycyrrhiza Uralensis

目的研究甘草总黄酮提取部位(GF)抗惊厥作用。方法 2017年6—8月课题组在宁夏医科大学药学院药理实验室根据预实验结果将实验分为对照组、GF各剂量组(400.0、150.0、50.0 mg/kg)及阳性对照组,小鼠灌胃给药7d后,于末次给药后1h实施实验。分别利用旷场实验(OFT)观察小鼠自主活动,研究GF可能的中枢作用;急性戊四氮(PTZ)、匹鲁卡品(PILO)及最大电休克致小鼠惊厥实验模型(MES),研究GF的抗惊厥作用。结果与对照组相比,在实验剂量范围内,GF不能影响小鼠自主活动;对小鼠PILO及MES引起的惊厥表现没有拮抗作用;与对照组比较,400.0 mg/kg剂量的GF能够显著延长PTZ诱导小鼠癫痫大发作的潜伏期(17±5)min、死亡时间(25±13)min和降低强制发作的百分率(46.7%)。150mg/kg剂量的GF能够延长PTZ诱导小鼠癫痫大发作的潜伏期(13±5)min、死亡时间(14±5)min及降低强制发作的百分率(66.7%)。结论 GF具有抗小鼠PTZ致惊厥作用。

Objective This paper tries to investigate the anticonvulsant effects of the flavonoid extract parts from glycyrrhiza uralensis（GF）. Methods The experiment was carried out in the pharmacological laboratory at college of pharmacy in Ningxia medical university in June to Augcst 2017. According to the results of preliminary experiment, male ICR mice were divided into the normal control group, flavonoids groups in different dosages（400.0, 150.0, 50.0 mg/kg） and positive control groups. The mice were intragastricaly ad-ministrated for seven days consecutively. The experiment was carried out on one hour after last administration. Open field test（OFT）was employed to observe the activity of the mice in study, the intended central nerve system（CNS） effects were analyzed. The acute pentylenetetrazole（PTZ） and pilocarpine（PILO） kindled seizure model, and maximal electroshock test（MES） were used to evaluate the anticonvulsive effect of GF. Results Compared with the control group, flavonoids had no excitation effects in CNS at the dose range of the test, and no antagonistic effects in the acute PILO kindled seizure and MES model. In the PTZ kindled seizure test, 400.0 mg/kg dose of GF could significantly delay the onset time of the tan tonic seizure of（17±5） min, postpone the onset of epileptic death of（25±13） min and decrease the mortality rate of the seizure of 46.7%. 150 mg/kg dose of GF could prolong the onset time of the tan tonic seizure of（13±5） min, delay the onset of epileptic death of（14±5） min and decrease the mortality rate of the seizure of 66.7%. Conclusion GF is effective of anticonvulsant activities of the mice.