响应面法优化紫果西番莲果胶多糖脱色工艺

Optimization of resin decoloration technology of pectin polysaccharide from Passiflora edulis Sims by response surface methodology

为优化紫果西番莲果胶多糖脱色工艺,通过静态吸附法从六种树脂中筛选出最佳脱色效果的聚酰胺树脂,随后通过单因素实验考察进样量、流速及pH对半乳糖醛酸保留率和脱色率的影响。采用Box-Behnken实验设计建立并分析了各因素分别与脱色率、半乳糖醛酸保留率之间的数学模型。结果表明,聚酰胺树脂对紫果西番莲果胶多糖最佳脱色工艺条件为:进样量3.5 BV,流速3 BV/h,pH3.5,脱色率为52.78%,达理论预测值的98.82%;半乳糖醛酸保留率85.62%,达理论预测值的98.19%。该法为进一步开发利用紫果西番莲果皮资源提供了科学依据。

The resin decoloration conditions of pectin polysaccharide from Passiflora edulis Sims was optimized by response surface methodology.The polyamide resin decolorization was optimized by static adsorption method from six kinds of resins,then the effects of loading quantity of sample,volume flow rate,pH on decolorization rate,galacturonic acid retention rate was studied by single test.The Box-Behnken was established to analyze the relationship between influening factors and decolorization rate,galacturonic acid retention rate of the polysaccharide respctively.The results showed that optimized conditions were sample loading was 3.5 BV,flow velocity was 3 BV/h,pH was 3.5.Under these conditions,a decolorization rate of 52.78% and galacturonic acid retention rate of 85.62% were obtained of which the actual values were 98.82% and 98.19% of the theoretical prediction values respectively. The method proposed in this study would provide a scientific base for further development and utilization of Passiflora edulis Sims source as well as functional food.