Streptomyces griseorubens C-5对堆肥木质纤维素降解及微生物群落代谢的影响

EFFECT OF Streptomyces griseorubens C-5 ON LIGNOCELLULOSE DEGRADATION AND MICROBIAL COMMUNITY METABOLIC OF COMPOST

为促进堆肥中木质纤维素降解，并了解其中微生物群落代谢功能多样性，主要采用Biolog—ECO平板法，研究添加灰略红链霉菌（Streptomyces griseorubens）C-5对堆肥中木质纤维素降解及微生物群落代谢能力的影响。结果显示：堆肥结束时，接菌处理的堆肥中木质素、纤维素和半纤维素的降解率分别比对照组（自然堆肥）提高29．94％、18．78％、12．77％；平均色度变化（AWCD）分析表明，接种处理可增加堆肥微生物的细胞代谢能力，尤其能提高堆肥中后期微生物群落对酯类和多聚物类碳源的代埘能力；主成分分析（PCA）结果显示，接种可提高微生物对氢基化合物、氨基酸和双亲化合物等碳源的代谢能力。

In order to promote lignocellulose degradation in compost and understand the diversity of metabolic function of microbial community, Biolog-ECO plate method was mainly used to study the effect of Streptomyces griseorubens C-5 on lignocellulose degradation and metabolic capacity of microbial communities in compost. The results showed that the degradation rate of lignin, cellulose and hemicellulose of the inoculated compost increase 29.94% , 18.78% and 12.77%, respectively compared with the control at the end of compost. The average well color development （AWCD） analysis showed that the cell metabolic capacity of microbial community is increased by Streptomyces griseorubens C-5 at thermophilic stage of the compost, especially the metabolic capacity of microbial community on ester and polymer carbon sources is increased in the mid-to late term of compost. The results of principal component analysis （PCA） showed that the metabolic capacity on carbon sources, such as amino compound, amino acids and amphiphile compounds of microorganisms in compost are enhanced by Streptomyces griseorubens C-5.