新疆野生樱桃李果皮中花色苷纯化工艺及体外抗氧化活性

Purification process and antioxidant activity of anthocyanins from Prunus cerasifera peel

目的:建立大孔吸附树脂HP2MGL纯化新疆野生樱桃李果皮中花色苷的方法,并检测樱桃李果皮中花色苷体外抗氧化活性。方法:比较10种大孔吸附树脂对樱桃李果皮中花色苷吸附解吸效果,用HP2MGL大孔树脂对樱桃李果皮中花色苷进行静态动态吸附及解吸条件优化,通过Box-Benhnken中心组合测试和响应面分析法确定最佳纯化条件,利用2种实验模型对樱桃李果皮中花色苷清除羟自由基及清除DPPH自由基进行研究,并与抗坏血酸进行比较。结果:HP2MGL大孔树脂是纯化新疆野生樱桃李果皮中花色苷较合适的大孔树脂类型;樱桃李果皮中花色苷在HP2MGL大孔树脂上吸附最佳时间为6 h,最佳纯化条件为乙醇体积分数80.8%,洗脱剂p H为2.5,洗脱剂流速为2.1 m L/min,理论最佳花色苷解吸率为86.7383%。在一定浓度范围内,樱桃李果皮中花色苷羟自由基清除能力、DPPH自由基清除能力与浓度呈正线性相关。结论:用响应面法确定最佳工艺合理,可用于樱桃李果皮中花色苷的纯化,且樱桃李果皮中花色苷浓度在一定范围之内具有较好的抗氧化活性。

Objective： To establish a method for the purification of anthocyanins in Prunus cerasifera peel by using macroporous adsorption resin HP2 MGL and detect the antioxidant activity of the extracted anthocyanins from Prunus cerasifera peel. Methods： The adsorption and desorption effects of 10 kinds of macroporous resins on anthocyanins from Prunus cerasifera peel were compared. The adsorption and desorption properties of resin HP2 MGL on anthocyanins from Prunus cerasifera peel were further investigated. The optimal purification conditions were determined by Box-Behnken center combination test and response surface analysis. Study on scavenging hydroxyl radicals and scavenging DPPH free radicals in anthocyanin from Prunus cerasifera peel by 2 kinds of experimental models and compared with ascorbic acid. Results： HP2 MGL macroporous resin was the best type of macroporous resin for the purification of anthocyanins in Prunus cerasifera peel. The best time to adsorb anthocyanins in HP2 MGL macroporous resin was 6 h. The optimum conditions for the purification of ethanol solution were 80.8%, the eluent p H was 2.5, the eluent flow rate was 2.1 m L/min. The scavenging ability of anthocyanin on hydroxyl radical and DPPH free radical were positively correlated with the concentration in the range of certain concentration. Conclusion： The optimum method was determined by the response surface method, and it could be used for the purification of anthocyanins from Prunus cerasifera peel. The anthocyanins of Prunus cerasifera peel has better antioxidant activity in the cetain concentration range.