摘要
目的:对蓝莓中的功能活性成分进行全面筛查。方法:首先建立一个含有200余种相关化合物的"待筛查化合物数据表"。然后使用超高效液相色谱-电喷雾-四级杆/飞行时间质谱(UPLC-ESI-Q-TOF/MS),分别在TOF/MS全扫描和Q-TOF/MS碎片扫描2种模式下分析蓝莓样品的功能活性成分。首先依据精确质量在"待筛查化合物数据表"中查出可能的化合物,再结合二级质谱的子离子碎裂特征对化合物进行定性分析。对于同分异构体,再根据色谱保留时间(出峰先后)、参考文献报道予以确定。为了解析各成分的二级质谱,对蓝莓中的主要成分花青素苷和黄酮苷的质谱裂解规律进行了探讨。结果:通过该方法灵敏、快速、准确地从市售蓝莓样品中鉴定出77个功能活性成分,其中包括34个花青素及其糖苷、18个黄酮及其糖苷、16个酚类化合物和9个维生素类化合物。结论:首次利用UPLC-ESI-Q-TOF/MS成功地对蓝莓中的功能活性成分进行了全面的定性分析。该分析方法方便快捷、灵敏度高、准确性好,有望将其推广至其他天然成分的研究之中。
Objective： To screen the functional active ingredients in blueberry without reference substance.Methods： First, a database of possible compounds was prepared including more than 200 compounds.Then, the blueberry sample was analyzed by TOF/MS full scanning mode and Q-TOF/MS fragments scanning mode using ultra performance liquid chromatography quadruple/time-of-flight mass spectrometry（UPLC-ESI-Q-TOF/MS）. According to the exact molecular weight, and the characteristics of ion fragmentation, the formula of each compound was qualitative confirmed. Results： Seventy-seven active ingredients was screened in blueberry, including 34 anthocyanin and their glycosides, 18 flavonoids and their glycosides, 16 phenolic compounds and 9 vitamin. At the same time, the MS fragmentation patterns of anthocyanin glycosides and flavonoid glycosides were discussed. Conclusion： UPLC-ESIQTOF/MS has been successfully used to identify the functional components in blueberry. It has high sensitivity and good accuracy. It is expected to be extended to other natural ingredients.
出处
《食品科技》
CAS
北大核心
2018年第2期286-292,共7页
Food Science and Technology
基金
2017年国家级大学生创新创业训练计划项目(201713705030)
国家自然科学基金项目(21572219)
