长链非编码RNA CRNDE检测在乳腺癌诊断中的意义

Value of detection of long non-coding RNA CRNDE in the diagnosis of breast cancer

目的探讨长链非编码RNA(lncRNAs)CRNDE在乳腺癌患者血浆中的表达及临床意义。方法采用实时荧光定量聚合酶链反应方法检测76例乳腺癌患者组织、远端癌旁组织及血浆lncRNA CRNDE的表达情况。分析血浆中lncRNA CRNDE表达与乳腺癌的临床病理特征关系,采用受试者工作特征曲线(ROC)评价血浆lncRNA CRNDE水平对乳腺癌的诊断效能。同时收集同期80例健康体检者为对照组检测其血浆中血浆lncRNA CRNDE表达情况。结果 lncRNA CRNDE在乳腺癌组织中的表达(2.73±1.66)明显高于癌旁组织(2.06±1.67),且在患者血浆中的表达水平(1.79±1.05)高于健康对照者(1.40±1.12),差异均有统计学意义(均P<0.05);血浆中lncRNA CRNDE的表达水平与患者临床病理特征无关(P>0.05);ROC曲线分析发现AUC为0.66。结论血浆中高表达的lncRNA CRNDE可作为乳腺癌诊断的一个潜在的新型生物标志物。

Objective To explore the expression of long non-coding RNA （lncRNA） colorectal neoplasia differentially expressed （CRNDE） in the plasma of breast cancer patients and its clinical significance. Methods Quantitative real-time polymerase chain reaction （qRT-PCR） was used to detect the expression of lncRNA CRNDE in breast cancer tissues, matched para-cancerous tissues and plasma from 76 patients with breast cancer. The correlation between lncRNA CRNDE expression in plasma and clinical pathological characteristics of breast cancer was analyzed. The receiver operating characteristic curve （ROC） was constructed to evaluate the efficiency of plasma levels of lncRNA CRNDE in the diagnosis of breast cancer. 80 physical examinees were simultaneously selected as the control group, and the expression of plasma lncRNA CRNDE was detected. Results The expression level of lncRNA CRNDE was significantly higher in cancer tissues than in matched para-cancerous tissues （（2.73±1.66） vs.（2.06±1.67）） as well as significantly higher in the patients with breast cancer than in the healthy controls （（1.79±1.05）vs. （1.40±1.12））, showing statistically significant differences （both P〈0.05）. There was no correlation between the expression level of lncRNA CRNDE in plasma and clinicopathological characteristics of breast cancer patients （P〉0.05）. The area under the ROC curve （AUC） was 0.66. Conclusion High expression of plasma lncRNA CRNDE may serve as a novel potential biomarker for the diagnosis of breast cancer.