圆尾鲎不同组织中河豚毒素的小鼠单位法分析及评价

Assessment of tetrodotoxin in different tissues of the horseshoe crab Carcinoscorpius rotundicauda by mouse unit method

目的了解福州、南平两地私售圆尾鲎不同组织受TTX污染的状况。方法采用小鼠单位法检测圆尾鲎的肌肉、卵和结缔组织中TTX的毒素量。结果在采集的10只圆尾鲎中,共有9只出现明显的TTX中毒症状。其中9只圆尾鲎的肌肉提取液致小鼠死亡、5只的卵提取液致小鼠死亡、6只的结缔组织提取液致小鼠死亡。结论圆尾鲎不同组织部位受TTX污染的状况不同,本批标本各组织中所含毒素量依次为肌肉>卵>结缔组织。

Objective To investigate the pollution situation of tetrodotoxin （TTX） in different tissues of the horseshoe crabCarcinoscorpius rotundicauda sampled from private stalls in Fuzhou and Nanping. Methods Mouse unit method was used to determine the TTX content in muscles, eggs and connective tissues of the horseshoe carbs. Results Among the 10 horseshoe carbs sampled, totally 9 showed significant symptoms of TTX poisoning. And mouse deaths were induced by muscle extract collected from 9 horseshoe carbs, egg extract from 5 ones and connective tissue extract from 6 ones respectively. Conclusions Different tissues of thehorseshoe carbs are contaminated differently by TTX, and the highest toxicity in this study is obtained from muscle samples, followed by egg and connective tissue ones.