摘要
目的:观察杜仲3种炮制品(盐炙烘制品、盐炙炒制品和生品)的木脂素类对大鼠肠系膜上动脉血管环的舒张作用,并探讨其相关机制。方法:使用Myograph动态描记系统,观察3种炮制品木脂素类对氯化钾溶液(60m M)、苯肾上腺素(PE)预收缩血管环的舒张作用;并分别用内皮途径抑制剂(鸟苷酸环化酶抑制剂[ODQ(10μM)]、一氧化氮合酶可逆抑制剂[L-NAME(100μM)]、环氧合酶抑制剂[Indo(5μM)])以及钾离子通道抑制剂(KV阻断剂4-AP、KCa阻断剂TEA、Kir阻断剂BaCl_2、KATP阻断剂Glib)处理血管,探讨杜仲木脂素(EUL)舒血管作用机制。结果:在杜仲的3种炮制品中,盐炙烘制品中提取的木脂素类舒血管作用最强,且对氯化钾溶液(60m M)和PE(10-5M)预收缩的血管都有浓度依赖性的舒张作用。另外,在内皮途径抑制剂中,L-NAME对杜仲木脂素类化合物(10-3g/ml)引起的舒张作用有显著的抑制作用,而ODQ和Indo则无影响;在钾离子通道抑制剂中,BaCl_2对杜仲木脂素类化合物(10-3g/ml)引起的舒张作用有显著的抑制作用,4-AP、Gli和TEA则无影响。结论:杜仲盐炙烘制品对大鼠肠系膜上动脉血管环舒张作用最强,其作用机制为杜仲木脂素通过内皮依赖途径(促进NO释放)和非内皮依赖途径(激活内向整流K+通道)促进肠系膜上动脉的舒张。
Objective: To investigate the vasorelaxant effect of extractives of three processed Eucommiae Cortex(EUL)(Baking and Stir products with salt,Crude products) on rat superior mesenteric arterial rings as well as its mechanisms. Methods: Isometric tensions of rat superior mesenteric arterial rings were recorded by a sensitive myograph system in vitro. We measured isometric tension changes of potassium chloride(KCl,60 m M),or phenylephrine(PE,10^-5 M) preconstricted rat superior mesenteric artery rings after the treatment of three processed Eucommiae Cortex at different concentrations,respectively. We also observed the vasorelaxant effect of Eucommiae Cortex on KCl(60 m M)-preconstricting rat superior mesenteric arterial rings after incubating with inhibitors of Endothelial mechanism,the endothelial NO synthase(e NOS) inhibitor L-NAME,the guanylate cyclase inhibitor ODQ and the cyclooxygenase inhibitor Indo,and inhibitors of Potassium ion channel,4-AP(KV channels blockers),and TEA(KCa channels blockers),and Ba Cl2(Kir channels blockers),and Glib(KATP channels blockers),respectively. The mechanism involved in vasodilatation induced by EUL was researched. Resnlts: The vasorelaxant effect of baking products with salt was best in three processed products. EUL(10^-3 g/ml) relaxed the superior mesenteric artery rings concentration-dependently by pre-contracted by KCl(60 m M) and PE. In inhibitors of Endothelial mechanism,the vasodilatation treated by EUL in KCl(60 m M)-preconstricted rat mesenteric artery,L-NAME had the obvious inhibitory action(P〈0. 05),but ODQ and Indo had no effect. In inhibitors of Potassium channel,the vasodilatation treated by EUL(10^-3 g/ml) in KCl(60 m M)-preconstricted rat mesenteric artery,Ba Cl2 had the obvious inhibitory action,but 4-AP,Gli,TEA had no effect. Conclusion: EUL(10^-3 g/ml) of baking products with salt could relax precontracted rat superior mesenteric arterial rings by KCl(60 m M),and its vasodilatation is strong. Its mechanisms are that EUL induces the relaxation in superior mesenteric arterial rings through an endothelium-dependent pathway(promoting NO release),and also through an endothelium-independent pathway(activating inward rectifying potassium channel blocker).
出处
《中药药理与临床》
CSCD
北大核心
2017年第5期98-103,共6页
Pharmacology and Clinics of Chinese Materia Medica
基金
陕西省教育厅重点科研计划项目(16JS096)
陕西省教育厅自然科学专项基金(NO.14Jk1630
NO.2013Jk0711)
西安医学院省级重点学科-药学(1007)
西安医学院药学省级重点学科建设项目(2016YXXK01
2016YXXK07
2016YXXK21)
关键词
杜仲
血管舒张
钾离子通道
一氧化氮
Processed of Eucommia cortex (杜仲)
vasodilatation
potassium ion channels
NO