中草药中4-香豆酸辅酶A连接酶的遗传进化分析

Genetic and Evolutionary Analysis of 4-Coumarate:CoA Ligase in Chinese Herbal Medicine

目的:研究中草药中4-香豆酸辅酶A连接酶(4CL)基因的遗传进化,为中草药中有效成分黄酮类物质等的有效积累和调控奠定理论基础。方法:运用ClustalW 1.83、Mega 5.0、CodonW、DNAMAN等软件对搜集的部分中草药中的4CL进行遗传进化树的构建、密码子偏好性分析及氨基酸保守区的分析。结果:不同植物以基因家族形式出现,通过遗传密码子使用情况分析,可推测植物中表达较强的基因。遗传进化分析表明,与模式植物相比,中草药中4CL基因有的聚在一起可能具有相同的功能,推测并没有发生基因的分化,而有些基因可能具有功能的分化。结论:通过4CL基因的遗传进化树、密码子偏好性分析、酶的保守区分析,可为进一步研究4CL在中草药中的应用提供理论支持。

Objective： To study the genetic evolution of 4CL gene in Chinese herbal medicine,and to lay a theoretical foundation for effective accumulation and regulation of flavonoids in Chinese herbal medicine.Methods： ClustalW 1.83,Mega 5.0,CodonW and DNAMAN etc were used to collect the part of Chinese herbal medicine in the 4CL for the construction of genetic evolution tree,codon preference analysis and amino acid conserved region analysis.Results： Different plants appeared in the form of gene family,and the expression of strong genes in plants could be inferred by using genetic codon usage analysis.Phylogenetic analysis showed that,compared with the model plant,Chinese herbal medicine 4CL gene may had the same function that did not have gene differentiation,while some had gene differentiation.Conclusion： Through the genetic evolution tree of 4CL gene,codon preference analysis and enzyme conserved region analysis,it will provide theoretical support for the further study of the application of 4CL in Chinese herbal medicine.