神经干细胞与神经元差异表达基因的生物信息学分析

Bioinformatics analysis of differentially expressed genes in neural stem cells and neurons

目的通过生物信息学技术探讨神经干细胞与神经元间基因表达的差异,找出关键的差异基因及其潜在的分子调控机制;并对其所涉及的功能进行分析预测。方法从GEO表达谱数据库中下载与神经干细胞及神经元相关的表达谱基因芯片数据系列GSE70171,导入基因芯片在线分析工具morpheus,筛选出神经干细胞和神经元之间表达差异的基因,并构建聚类分析热图。采用DAVID数据库进行GO功能分析和KEGG通路富集分析,并使用STRING分析与Cytoscape软件构建PPI网络。结果筛选出表达差异的基因有4 022个,其中神经干细胞比神经元上调的基因有2 146个,下调基因1 876个。对表达差异的基因进行的生物信息学分析发现,下调的基因主要与神经元功能相关,上调基因与神经干细胞细胞再生和有肿瘤特征相关,其中神经干细胞的细胞周期通路和癌症通路存在研究价值。结论神经干细胞与神经元之间存在表达差异基因。几个关键基因CDC6、CDKN2A、CDC14A、BUB1、TTK、CHEK1、CDC25C在细胞周期通路中可能与神经干细胞再生功能相关。而KIF23、CDKN2A、TNC、CDC25C、CDCA5、BRCA1可能与神经干细胞的肿瘤特征相关。然而,这些关键基因的功能还需要今后的实验研究进一步证实。

Objective Through the bioinformatic technique,the differences in gene expression between neural stem cells and neurons were explored. The key genes and their potential molecular regulation mechanisms were identified. The functions involved were analyzed and predicted. Methods The gene series GSE70171 related to neural stem cells and neurons were downloaded from the GEO expression profile database. Firstly,the genes were analyzed by gene chip online analysis tool Morpheus. Then among these genes,genes that differ in expression between neural stem cells and neurons were screened out. Finally,expression differences in gene construction cluster analysis heat map. DAVID database was used to perform GO functional analysis and KEGG pathway enrichment analysis. The STRING analysis and Cytoscape software were used to build PPI network.Results There were 4022 genes screened out,among which neurons had more 2 146 genes downregulated and 1 846 genes up-regulated than neural stem cells. The bioinformatic analysis of the genes expressing the differences revealed that the down-regulated genes were mainly related to neuronal function,and the up-regulation genes were related to the regeneration of neural stem cells and the neoplasms characteristics. The cell cycle pathway and the microRNAs pathway of neural stem cells were of great value. Conclusions The result is a differential gene between neural stem cells and neurons. Several key genes,such as CDC6,CDKN2 A,CDC14 A,BUB1,TTK,CHEK1,CDC25C,may be related to the regeneration function of neural stem cells in the cell cycle pathway. And KIF23,CDKN2A,TNC,CDC25C,CDCA5,BRCA1 may be related to the neoplasms characteristics of neural stem cells. However,the function of these key genes requires further confirmation in future experimental studies.