福建南平地区猪场饮用水中肠球菌耐药特性研究

Study on the antibiotic resistance of Enterococcus from drinking water of pig farms in Nanping District of Fujian Province

旨在探讨不同猪场来源的饮用水肠球菌对6种抗菌药的耐药情况,了解南平地区猪场水源与饮水器出水在肠球菌耐药表型及耐药谱方面的差异。水样中富集培养肠球菌,通过生化、PCR方法鉴定肠球菌菌株。微量肉汤稀释法开展药物敏感性试验,测定肠球菌对抗菌药的最小抑菌浓度（MIC）。结果显示：南平地区11个猪场46株饮用水分离菌对抗菌药的耐药率由高到低分别为：红霉素〉土霉素〉氯霉素〉环丙沙星〉氨苄西林〉万古霉素,对前4种药的耐药率分别为：84.78%、76.09%、67.39%和50%,对氨苄西林的耐药率较低,为19.57%,分离出2株耐万古霉素肠球菌（VRE）,耐药率为4.35%。蓄水池分离菌总体表现为对土霉素、红霉素、氯霉素耐药,耐药率较饮水器饮用水分离菌略低,为72.73%、72.73%、63.64%,对环丙沙星、氨苄西林的耐药显著低于饮水器分离菌,分别为27.27%、9.09%。保育舍饮水器分离菌对所有药物都有更高的耐药率,其次为育肥舍、哺乳舍、怀孕舍饮水器。南平地区11个猪场一共有13种耐药谱,有19株分离菌对4~5种的抗菌药耐药,其中5株来自于保育舍饮水器。水池进水口及蓄水池分离菌的多重耐药率均低于饮水器分离菌,保育舍饮水器多重耐药率最高。研究结果提示,猪场源头饮用水与饮水器饮用水分离菌对抗菌药耐药存在差异,饮水管网内耐药菌的存在可能是主要原因。

This study was designed to explore the resistance of Enterococcus from drinking water of different pig farms and investigate the difference of resistant phenotype and resistant spectrum between original water and pipe network water in Nanping District of Fujian Province.Water samples were collected from 11 pig farms of Nanping District.Enterococcus were isolated and identified by biology-chemical and PCR methods.Micro-Broth methods recommended by CLSI were used to detect the MIC of Enterococcus.The result showed that the resistant ratio of 46 strains of Enterococcus isolated from 11 pig farms to oxytetracycline,erythrocin,chloromycetin,ciprofloxacin,ampicillin and vancomycin was 84.78%,76.09%,67.39%,50%,19.57% and 4.35%,respectively.The isolates from original water samples showed resistance to oxytetracycline,erythrocin and chloromycetin in general,which was 72.73%,72.73% and 63.64%,respectively,a little lower than samples from pipe network water.They showed much lower resistant ratio to ciprofloxacin and ampicillin than samples from pipe network.Pipe network water samples from weaning pig house showed the highest resistant ratio.There were 13 kinds of Enterococcus resistant spectrums found from 11 pig farms.There were 19 strains of Enterococcus showed resistance to 4 to 5 kinds of antimicrobials,while,5 of them came from the weaning pig house.Original water samples showed lower multi-drugs resistant ratio than pipe network water samples,and weaning pig house samples showed the highest multi-drugs resistant ratio.Our finding suggested that there was a significant difference in the resistance of Enterococcus from original water and pipe network water,which might be caused by the resistant bacteria in pipe network.