补阳还五汤对大鼠急性上颈脊髓损伤后血小板活化因子的影响

Effect of Buyanghuanwu(补阳还五)decoction on the expression of platelet activating factor after acute spinal cord injury in rats

目的 :观察补阳还五汤对大鼠急性上颈脊髓损伤(SCI)模型大鼠脊髓组织血小板活化因子(DAF)表达的影响。方法:将SPF级别3月龄Wistar大鼠60只,雌性,随机分成假手术组、模型组、甲强龙组、补阳还五汤组(即中药组),每组15只。造模后第1天,甲强龙组经尾静脉注射治疗共24 h,首剂量30 mg/kg,之后剂量5.4 mg/kg·h,每4 h给药1次;中药组以中剂量补阳还五汤颗粒剂,配制成含颗粒剂2.00 g/ml的水溶液,每日3.5 g/kg灌胃,相当于成人用量的1倍;模型组和假手术组予等体积生理盐水灌胃,均每日2次,用药2周。治疗后第1、3、7、14天分别以BBB分级法评价神经功能恢复情况;采用双抗体夹心法(ELISA法)于术后1、7、14 d检测脊髓损伤节段PAF的表达。结果:治疗后第1天,模型组、甲强龙组和中药组BBB评分明显降低,与假手术组比较差异有统计学意义(P<0.01),但3组间比较差异无统计学意义(P>0.05)。治疗后第7、14天评分结果,中药组与甲强龙组大鼠四肢运动功能评分明显高于模型组(P<0.01),而中药组与甲强龙组比较差异无统计学意义(P>0.05)。治疗后7、14 d,中药组与甲强龙组大鼠PAF值与模型组比较明显下降(P<0.01),而中药组与甲强龙组比较差异无统计学意义(P>0.05)。结论:急性上颈脊髓损伤后以补阳还五汤治疗,可以改善SCI大鼠四肢运动功能,其作用机制可能与通过降低脊髓组织中PAF表达明显有关。

Objective：To investigate the effect and mechanism of Buyanghuanwu decoction on platelet activating factor expression in spinal cord tissue of model of acute upper cervical spinal cord injury in rats. Methods：Sixty SPF grade 3-month-old female Wistar rats were randomly divided into sham operation group,model group,methylprednisolone group and Buyanghuanwu decoction（Traditional Chinese Medicine group,TCM）,with 15 rats in each group. The first day after the modeling,the methylprednisolone group were treated by injection of the tail vein for a total of 24 h,the first dose of 30 mg/kg,followed by a dose of 5.4 mg/kg·h,and 1 time per 4 h. The traditional Chinese medicine group was prepared with a medium dose of Buyanghuanwu decoction granules which were prepared into a solution containing 2 g/ml of granules,3.5 g/kg per day gavage,was equivalent to 1 time the amount of adult consumption. The model group and the sham operation group were given equal volume of normal saline for 2 times a day for 2 weeks. The recovery of nerve function was evaluated by BBB classification at 1,3,7,14 days after treatment. The expression of PAF in the segment of spinal cord injury was detected by double antibody sandwich（ELISA） method at 1,7,and 14 d postoperatively. Results：At the first day after treatment,BBB score in model,TCM and methylprednisolone groups were lower than that of sham operation group（P〈0.01）,but there was no difference among the three groups（P〈0.05）. At 7,14 days afer treatment,BBB score in TCM and methylprednisolone groups were higher than that of model group significantly（P〈0.01）; but there were no significant difference between TCM group and methylprednisolone group（P〈0.05）. PAF expression in TCM group and methylprednisolone group were lower than that of model group at 7,14 day afer treatment significantly（P〈0.05）; but there were no significant difference between TCM group and methylprednisolone group（P 0.05）. Conclusion：Buyanghuanwu decoction treatment after acute upper cervical spinal cord injury can significantly improve locomotor recovery by inhibiting the expression of PAF.