非小细胞肺癌组织miRNA-34b/c甲基化及预后相关性

Methylation of miRNA-34b/c in non-small cell lung cancer and correlation with prognosis

目的探讨miRNA-34b/c在可手术的非小细胞肺癌组织中的甲基化及其与预后的相关性。方法回顾性分析中国医科大学附属盛京医院2009年12月-2015年12月期间行手术治疗的53例非小细胞肺癌患者的临床资料。采用定量RT-PCR检测上述病例肺癌标本中miRNA-34b/c的甲基化水平,应用Kaplan-Meier方法进行单因素生存曲线分析miRNA-34b/c甲基化水平与非小细胞肺癌生存的相关性,Log-rank法进行检验,采用Cox风险比例模型分析预后的独立影响因素。结果非小细胞肺癌miRNA-34b/c甲基化水平较高,生存分析显示miRNA-34b/c甲基化组生存期明显低于非甲基化组(P<0.01),Cox风险比例模型分析显示miRNA-34b/c甲基化可作为非小细胞肺癌患者预后的独立影响因素。结论非小细胞肺癌miRNA-34b/c甲基化水平高,miRNA-34b/c甲基化提示非小细胞肺癌患者预后不良。

Objective To investigate the methylation of miRNA-34b/c in non small cell lung cancer（NSCLC） and its correlation with prognosis. Methods The clinical data of 53 cases of non small cell lung cancer treated by surgery in Shengjing affiliated hospital of China Medical University from December 2009 to December 2015 were retrospectively analyzed. The methylation level of miRNA-34b/c in lung cancer tissues was detected by quantitative RT-PCR. Using Kaplan-Meier method to analyze the relationship between miRNA-34b/c methylation and survival of non-small cell lung cancer, independent factors of prognosis were analyzed by Cox proportional hazards model. Results The methylation level of miRNA-34b/c in NSCLC was much higher（P〈0.05）, the survival time of miRNA-34b/c was significantly shorter in methylation group than in non-methylation group（P〈0.01）. Cox proportional hazard model analysis showed that the methylation of miRNA-34b/c could be used as an independent factor for the prognosis of patients with non-small cell lung cancer. Conclusion Methylation of miRNA-34b/c is related to non small cell lung cancer, might be an index for poor prognosis in patients with small cell lung cancer.