天山假狼毒不同部位挥发性成分比较分析研究

Comparative Analysis of Volatile Components in Different Parts of Stelleropsis Tianschanica

目的:用GC-MS分析比较天山假狼毒叶、花和根中挥发性成分。方法:采用索氏提取法提取天山假狼毒叶、花和根中的挥发性成分,经GC-MS联用技术对挥发性成分进行分析鉴定,采用色谱峰面积归一化法计算各挥发性成分的含量。结果:结果共鉴定出179个挥发性成分,其中天山假狼毒叶中81个,相对含量占总挥发性成分总量的82.77%;花中108个,相对含量占总挥发性成分总量的82.85%;根中112个,相对含量占总挥发性成分总量的85.98%;三者共同含有挥发性成分33个,共有成分在叶子中占总挥发性成分的39.24%,在花中占总挥发性成分的35.86%,在根中占总挥发性成分的48.89%。其中(Z,Z)-9,12-十八碳二烯酸在天山假狼毒叶、花、根挥发性成分中相对含量都最高,分别为11.12%、9.8%、22.49%。结论:天山假狼毒叶、花、根挥发性成分接近,但在组分和含量上存在一定的差异。

Objective： To compare and analyze the chemical constituents of volatile oils extracted from the different parts（ flowers,leaves and roots） of Stelleropsis tianschanica by chromatography-mass spectrometry（ GC-MS）. Methods： The volatile oil was extracted by diethyl ether-Soxhlet extraction method and analyzed by GC-MS with a capillary gas chromatographic column. The relative contents of the volatile compounds were calculated by chromatographic peak area normalization method. Results： Totally 179 volatile constituents in the different parts of Stelleropsis tianschanica were identified. Among them,81 compounds were identified in leaves,and the relative content accounted for 82. 77% of the total volatile compounds; 108 compounds were identified in flowers,and the relative content accounted for 82. 85% of the total volatile compounds; 112 compounds were identified in roots,and the relative content accounted for 85. 98% of the total volatile compounds. Totally 33 compounds existed in all the three parts,and the content accounted for39. 24% of the total volatile components in leaves,35. 86% in flowers and 48. 89% in roots. The relative content of（ Z,Z）-9,12-octadecadienoic acid in leaves,flowers and roots of S. tianschanica was the highest,which accounted for 11. 12%,9. 8% and 22. 49%,respectively. Conclusion： The different parts of S. tianschanica have similar volatile components,while the specific substances and the contents are different.