生肌化瘀膏对小鼠创面修复期肉芽组织总IL-1β和Ⅰ、Ⅲ型胶原的影响

Effects of Shengji Huayu Ointment on Total IL-1β,Collagen Type Ⅰ and Collagen Type Ⅲ in Granulation Tissue of Mice in Wound Repair Period

目的:探讨生肌化瘀膏促进创面修复的作用机制。方法:将Db/db小鼠采用随机区组设计分为生肌化瘀膏高(15 g·kg^(-1))、中(10 g·kg^(-1))、低(5 g·kg^(-1))剂量组、阳性对照组(贝复济)、模型对照组,正常对照组采用balb/c小鼠,每组10只。采用小鼠皮肤全层缺损创面为模型,采用免疫组化和图像分析相结合的方法,观察各组小鼠创面修复期肉芽组织中HE染色、IL-1β和Ⅰ、Ⅲ型胶原的变化。结果:HE染色显示生肌化瘀膏中、高剂量组创面愈合明显;各组IL-1β的表达均不明显;生肌化瘀膏各剂量组的Ⅰ型和Ⅲ型胶原水平均高于模型对照组(P<0.05)。结论:生肌化瘀膏可以促进小鼠创面修复,减少瘢痕形成。

Objective： To investigate the mechanism of promoting wound healing of Shengji Huayu ointment. Methods： Db/db mice were divided into 5 groups according to the randomized block design： three Shengji Huayu ointment groups respectively at high（ 15 g·kg^（-1））,middle（ 10 g·kg^（-1）） and low（ 5 g·kg^（-1）） dose,the positive control group（ Beifuji） and the model control group,and balb/c mice were used in the normal control group,and each group was with 10 mice. The model of full-thickness skin defect in mouse skin was used,and after HE staining,IL-1β and collagen type Ⅰ and type Ⅲ in granulation tissue were observed by immunohistochemistry and image analysis. Results： HE staining showed that the wound healing was obvious in the high dose group and the middle dose group. The expression of IL-1β in each group was not obvious. The levels of collagen type Ⅰ and type Ⅲ in the three Shengji Huayu ointment groups were higher than those in the model control group（ P 0. 05）. Conclusion： Shengji Huayu ointment can promote wound healing and reduce scar formation in mice.