细胞黏附因子1在子宫内膜异位症患者中的表达及临床意义

Expression of cell adhesion factor-1 in endometriosis

目的收集子宫内膜异位症患者的异位组织、在位组织和正常子宫内膜组织,探讨细胞黏附因子1(CADM1)表达水平的变化。方法通过采用免疫组化法对45例子宫内膜异位症患者的异位组织、在位组织和30例正常子宫内膜组织的CADM1表达水平进行分析,并采用Western印迹法进行检测,染色结果通过全自动彩色图像分析系统(HMIAS-2000)进行染色强度和阳性率判定。结果 CADM1在子宫内膜异位组织、在位组织和正常组织中的阳性表达差异具有统计学意义(P<0.05),Western印记条带中CADM1的灰度值差异具有统计学意义(P<0.05)。结论 CADM1在子宫内膜组织异位发生发展过程中存在表达下调或者失活的现象,CADM1表达水平的变化对临床诊断和治疗子宫内膜异位症具有可参考的临床意义。

Objective To investigate the expression and clinical significance of CADM1 in the ectopic and eutopic endometrium in endometriosis patients.Methods The expression of CADM1 protein in ectopic and entopic endometrium tissues from 45 patients with EMs and endometrium tissues from 30 patients with normal endometrium from May 2014 to March 2015 were explored by immunohistochemistry. They were detected by Western imprinting method. The hmias-2000 automatic color image analysis system was used to determine the staining intensity and positive rate.Results Differences of CADM1 in endometriosis group, positive expression in tissue and normal tissue were significant （P〈0.05）. Differences among Western mark CADM1 gray value contrast results were significant （P〈0.05）.Conclusion CADM1 of endometrial tissue has a expression of downregulation or inactivation in the process of developing the ectopic. Change of CADM1 expression can provide reference for clinical diagnosis and treatment of endometriosis.