半胱胺修饰的FePt纳米颗粒对HeLa细胞的毒性

Cytotoxicity of Cysteamine Modified FePt Nanoparticles in HeLa Cells

为探究半胱胺修饰的FePt纳米颗粒的抗肿瘤特性,本文采用化学还原法,制备出直径约3nm的FePt纳米颗粒,再用半胱胺(cysteamine,Cys)重新修饰其表面,将得到的FePt-Cys纳米颗粒分别与HELF(人胚肺成纤维细胞)和HeLa细胞(人宫颈癌细胞)孵育,用CCK-8法评估其细胞毒性,并通过流式细胞仪分析其对两种细胞周期分布的影响.结果发现,在一定浓度范围内,FePt-Cys纳米颗粒可以显著抑制HeLa细胞的增殖,但不影响HELF细胞的生长,其机制可能是通过细胞周期调节作用来产生细胞毒性.基于其对肿瘤细胞显著的杀伤作用和对正常细胞良好的生物相容性,FePt-Cys纳米颗粒将来可能作为一种有效的化疗药剂.

In order to investigate the anti-tumor properties of cysteamine（Cys）modified FePt nanoparticles（NPs）,a chemical reduction method was implemented to synthesize the 3 nm NPs.With cysteamine remodified,the cytotoxicity of FePt-Cys NPs was studied by CCK-8 assay in HELF（human embryonic lung fibroblast cells）and HeLa cells（human cervical cancer cells）.In addition,to explore the detailed cytotoxic mechanism,cell-cycle redistribution analysis was conducted in both cell lines.The results indicate that FePt-Cys NPs could cause significant cytotoxicity in HeLa cells by cell-cycle regulation,but show nontoxicity to HELF cells below a proper concentration in the meantime.Given the remarkable inhibiting effect in tumor cells while a good biocompatibility to normal cells,FePt-Cys NPs could serve as an effective agent in future chemotherapy.