酶联免疫吸附法快速检测婴儿配方奶粉中乳铁蛋白含量

Rapid detection of lactoferrin content in infant formula by enzyme-linked immunosorbent assay

目的采用乳铁蛋白快速检测试剂盒,通过酶联免疫吸附法定量测定婴儿配方奶粉中的乳铁蛋白含量,并验证该方法的准确性、稳定性和可靠性。方法按照试剂盒操作说明制作标准曲线,选取乳铁蛋白阴性样品进行添加回收实验,验证方法的准确性;选取一个乳铁蛋白阳性样品,重复检测6次,计算结果偏差,验证方法的稳定性;选取12个乳铁蛋白阳性样品,分别采用试剂盒方法和高效液相色谱法进行检测,对比2种方法的检测结果,验证试剂盒方法的可靠性。结果试剂盒检出限为2 mg/100 g;试剂盒检测方法回收率在101.6%~109%;相对标准偏差(relative standard deviation,RSD)为5.34%;试剂盒方法检测结果与标签值的偏差为-6.90%~6.45%,和高效液相色谱法检测结果的偏差为-6.45%~6.9%,说明试剂盒方法具有很好的可靠性。结论乳铁蛋白试剂盒检测方法灵敏度高、操作简单,能快速准确地测定婴儿配方奶粉中的乳铁蛋白含量。

Objective To detect the content of lactoferrin in infant formula by enzyme-linked immunosorbent assay with lactoferrin rapid detection kit, and verify the accuracy, stability and reliability of the method. Methods The standard curve was made according to the instruction of the kit. The lactoferrin negative sample was selected for the spiking recovery experiment to test the accuracy of the method. A lactoferrin positive sample was repeated 6 times to verify the stability of the method. Twelve lactoferrin positive samples were selected and tested by the kit and HPLC. The test results of the 2 methods were compared to verify the reliability of the kit method. Results The limit of detection of the kit was 2 mg/100 g. The recovery rate of the kit method was 101.6%-109%. The relative standard deviation was 5.34%. The deviation between the kit result and the label value was -6.90%-6.45% and the deviation between the kit result and the HPLC result was -6.45%~6.9%, which indicated that the kit method had good reliability. Conclusion Lactoferrin kit detection method has high sensitivity and simple operation, which can quickly and accurately determine the lactoferrin content in infant formula.