清肠温中方对溃疡性结肠炎大鼠干扰素γ诱导蛋白10的影响

Study on Effect of Qing-Chang Wen-Zhong Decoction on Interferon Gamma Induced Protein 10 in Ulcerative Colitis Rats

目的:研究清肠温中方对溃疡性结肠炎大鼠结肠组织干扰素γ诱导蛋白10(IP10)的影响。方法:自由饮用4.5%DSS溶液7天制备UC模型,同时给予清肠温中方低剂量、中剂量、高剂量及美沙拉嗪(莎尔福)进行灌胃干预,处死大鼠,取结肠组织,应用酶联免疫吸附法检测结肠白介素-1α(IL-1α)、IL-1β、IL-6、肿瘤坏死因子-α(TNF-α)、γ-干扰素(INF-γ)的表达水平,免疫组织化学法检测IP10的蛋白表达及分布。结果:与正常组相比,DSS诱导的UC大鼠结肠炎症因子(IL-1α、IL-1β、IL-6、TNF-α、INF-γ)和IP10的表达水平均显著升高,而经过7天的干预后,结肠炎症因子(IL-1α、IL-1β、IL-6、TNF-α、INF-γ)和IP10的表达水平明显降低(p<0.01,p<0.05)。结论:清肠温中方能够通过抑制IP10的表达,下调结肠炎症因子(IL-1α、IL-1β、IL-6、TNF-α、INF-γ)的水平,从而抑制肠道抑制炎症、修复肠粘膜损伤,达到治疗溃疡性结肠炎的目的。

This paper was aimed to study the effect of Qing-Chang Wen-Zhong（QCWZ） decoction on interferon gamma induced protein 10（IP10） in colon tissues of rats with ulcerative colitis（UC）. The UC model was induced using 4.5% DSS added to distilled water for 7 days. At the same time, low-, medium-and high-dose of QCWZ decoction and mesalazine was given by gavage route daily. Then, the rats were killed and the colon tissues were taken. Expression level of interleukin-1 alpha（IL-1α）, IL-1β, IL-6, tumor necrosis factor alpha（TNF-α） and interferon gamma（INF-γ） in colon were detected by Elisa assay. The expression and distribution of IP10 protein were detected by immunohistochemistry（IHC）. The results showed that compared with the normal group, inflammatory factors（IL-1α, IL-1β, IL-6, TNF-α, INF-γ） and IP10 expression level in DSS-induced UC rats were significantly increased. After 7 days of intervention,inflammatory factors（IL-1α, IL-1β, IL-6, TNF-α, INF-γ） and IP10 decreased significantly（p〈0.01, p〈0.05）. It was concluded that QCWZ decoction may down-regulate the expression of IP10 and inflammatory factors（IL-1α, IL-1β, IL-6, TNF-α, INF-γ）, and then inhibit intestinal inflammation and repair intestinal mucosal damage, so as to achieve the purpose of UC treatment.