蔷薇花抑制酪氨酸酶活性成分提取工艺研究

Research on Extraction Process of Tyrosinase Inhibitor in the Rose multiflora

以左旋多巴为底物,研究蔷薇花提取物对酪氨酸酶的抑制。研究提取溶剂、提取料液比、提取温度和提取时间等因素对蔷薇花提取液抑制酪氨酸酶的影响,在单因素的基础上采用正交试验确定蔷薇花提取液的最佳提取工艺。试验结果显示,蔷薇花提取液对酪氨酸酶有显著的抑制作用,最佳提取条件为:提取溶剂50%乙醇溶液、料液比1︰20 g/m L、提取温度20℃、提取时间10 min,此时测得的蔷薇花提取液对酪氨酸酶的抑制率为87.32%,抑制酪氨酸酶的IC50值为2.269 mg/m L。

Study the extract from Rosa multiflora on inhibition of tyrosinase with L-dopa as the substrate. The extraction solvent, extraction material-liquid ratio, extraction temperature, extraction time and other factors were investigated based on its tyrosinase inhibition. The best extraction process condition for Rosa multiflora extract was optimized by orthogonal optimization design based on single factor. The results indicated that extract from Rosa multiflora had very significant inhibitory effect on tyrosinase, The best process condition was using 50% ethanol-water solvent, material-liquid ratio of 1 ： 20 g/mL, extracting 10 min under 20 ℃. The tyrosinase inhibition rates under this condition was 87.32%, and IC50 for diphenolase of tyrosinase inhibition was 2.269 mg/mL.