HERV-HLTR相关蛋白2在肝癌中的表达及其临床意义

Expression and clinical significance of HERV-HLTR-associating protein 2 in hepatocellular carcinoma

目的检测肝细胞癌组织中HERV-HLTR相关蛋白2(HHLA2)的表达及其与不同病理分级、临床分期、肝功能及甲胎蛋白(AFP)的关系。方法采用免疫组化法检测150例肝癌石蜡包埋组织标本中HHLA2的表达阳性率,并以10例患者正常肝脏组织标本作为正常对照,比较在不同的病理分级、临床分期、肝功能分级及AFP水平情况下肝癌组织中HHLA2的表达差异。结果肝癌组织中HHLA2的阳性表达率显著高于正常对照(P<0.05),低分化肝癌HHLA2表达阳性率最高,低分化、中分化肝癌组HHLA2表达阳性率显著高于高分化组(P<0.05);肝癌巴塞罗那分期C期、D期患者HHLA2阳性表达率显著高于A期、B期(P<0.05);肝功能Child-Pugh A级的患者HHLA2阳性表达率明显低于B级及C级(P<0.01);不同年龄、不同血清AFP水平患者肝癌组织中HHLA2的表达率差异无统计学意义(P>0.05)。结论肝癌细胞癌患者癌组织中HHLA2的阳性表达率明显升高,HHLA2可能在肝癌的发生、发展、转移中具有一定的作用。

Objective To investigate the expression of HERV-HLTR-associating protein 2（HHLA2）in hepatocellular carcinoma（HCC）tissue and its relationship with different pathological grades,clinical stages,liver function and serum AFP level.Methods The positive rate of HHLA2 expression in 150 cases of paraffin-embedded HCC tissues was detected by immunohistochemistry.Ten cases of normal liver tissue specimens from health donors served as the normal control.The differences of HHLA2 expression in HCC tissues were compared among different pathological grades,clinical stage,liver function grades and serum AFP levels.Results The positive expression rate of HHLA2 in HCC tissue was significantly higher than that in normal control（P0.05）.The positive rate of HHLA2 expression in low differentiated HCC tissue was highest.The positive rate of HHLA2 expression in the low differentiation and moderate differentiation HCC groups was significantly higher than that in the high differentiation group（P0.05）.The positive expression rate in the patients with Barcelona stage C and D was significantly higher than that in the patients with the stage A and B（P0.05）.The positive expression rate in the patients with Child-Pugh stage A was significantly higher than that in the patients with stage B and C（P0.01）.While the HHLA2 expression rate had no statistically significant difference among different ages and different serum AFP levels（P0.05）.Conclusion The positive expression rate of HHLA2 in cancer tissues of HCC patients is significantly increased.HHLA2 might play a certain role in the occurrence,development and metastasis of HCC.