低盐饮食对高果糖引起血管重构的保护作用

Protective effect of low salt diet on vascular remodeling induced by high-fructose

目的探究低盐饮食对高果糖引起血管重构的保护作用。方法选取Wistar雄性大鼠42只,体质量180~200g,共饲养8周,随机分6组:(1)对照组,给予正常大鼠饲料及蒸馏水;(2)高果糖组(HF组),给予正常饲料(0.5%NaCl,w/w)及果糖水(10%,w/v);(3)高盐组(HNa组),给予高盐饲料(7%NaCl,w/w)和蒸馏水;(4)高果糖联合高盐组(HFNa组),给予高盐饲料同时给予10%果糖水;(5)高果糖联合低盐组(HFLNa组),给予低盐饲料(0.07%NaCl,w/w)同时给予10%果糖水;(6)高果糖联合螺内酯组(HFE组),给予10%果糖水4周后,加入HFE(50mg·kg^(-1)·d^(-1)管饲法),再持续4周。检测各组大鼠动脉血压、血管壁组织学评价,以及α-SMA、纤连蛋白等在血管壁的表达变化。结果 (1)与对照组血压(111.03±9.17)mm Hg相比,HF组(133.94±5.86)mm Hg与HNa组(128.09±7.56)mm Hg血压均显著升高(P<0.05);(2)HF主要引起血管壁中层平滑肌增生,HF组α-SMA的表达结果(0.006 3±0.000 2)与对照组(0.004 6±0.000 3)相比明显增加(P<0.05),且弹力纤维增多;而HNa则主要刺激弹力纤维增粗,纤连蛋白在HNa组(0.002 6±0.000 2)和HF组(0.004 7±0.000 2)的表达与对照组(0.001 3±0.000 1)相比显著增多(P<0.001);(3)HFLNa组(106.04±9.59)mm Hg和HFE组(103.99±7.12)mm Hg血压与HF组血压相比明显降低(P<0.05);且HFLNa组(0.006 8±0.000 2)和HFE组(0.004 2±0.000 4)的血管重构得到改善,α-SMA表达与HF组(0.006 3±0.000 2)相比显著减少(P<0.05)。结论低盐饮食可有效改善HF引起的血管重构。

Objective To investigate the effect of low salt diet on vascular remodeling of rat induced by high fructose（HF）.Methods Wistar male rats weighed 180-200 g were fed for 8 weeks and randomly divided into 6 groups：（1）control group was given the normal fodder and distilled water;（2）high fructose group（HF）was given normal fodder（0.5% NaCl,w/w）and fructose water（10%,w/v）;（3）high-salt group（HNa）was given high salt fodder（7%NaCl,w/w）and distilled water;（4）high fructose combined with high salt diet group（HFNa）was simultaneously given high salt fodder and 10% fructose water;（5）high fructose combined low salt group（HFLNa）was simultaneously given low salt fodder and 10% fructose water;（6）high fructose combined with spirotaclone group（HFE）was given 10%fructose water for 4 weeks and then added with spirotaclone（50 mg·kg^（-1）·d^（-1） by tube feeding）for continuous 4 weeks.The changes of arterial blood pressure,vascular wall histological evaluation and expression ofα-SMA and fibronectin in vascular wall were detected in each group.Results（1）Compared with the blood pressure[（111.03±9.17）mm Hg]in the control group,the blood pressure in the HF and HNa groups were（133.94±5.86）mm Hg and（128.09±7.56）mm Hg respectively,which were significantly increased（P0.05）;（2）HF mainly caused the hyperplasia of vascular wall middle layer smooth muscle.Theα-SMA expression results in the HF group was（0.006 3±0.000 21）,which in the control group was（0.004 6±0.000 31）,the difference was statistically significant（P0.05）,moreover which promoted the elastic fibers increase;while HNa mainly stimulated the elastic fibers to thicken and extracellular matrix deposition,the fibronectin expression was 0.002 6±0.000 2 in the HNa group and（0.004 7±0.000 2）in the HF group,compared with（0.001 3±0.000 1）in the normal group,which were significantly increased（P0.001）;（3）the blood pressure was（106.04±9.59）mm Hg in the HFLNa group,（103.99±7.12）mm Hg in the HFE group,compared with（133.94±5.86）mm Hg in the HF group,showing that the blood pressure in the HFLNa group and HFE group was significantly decreased compared with the HF group（P0.05）;moreover the vascular remodeling in the HFLNa group（0.006 8±0.000 2）and HFE group（0.004 2±0.000 4）was improved,and compared with the HF group（0.006 3±0.000 2）,α-SMA expression was significantly decreased（P0.05）.Conclusion Low salt diet can effectively improve vascular remodeling induced by HF.