中国女子长跑运动员GLUT1基因rs841853位点多态性与运动能力之间的关联性

Association Between GLUT1 rs841853 Polymorphism and Sports Performance in Chinese Female Long Distance Runners

目的:探讨葡萄糖转运蛋白1(glucose transporter 1,GLUT1)基因rs841853 G/T多态位点作为评估和筛选长跑运动能力遗传标记的可行性。方法:于2009—2010年选取78名中国女子专业长跑运动员,采用基质辅助激光解吸附电离飞行时间质谱检测技术解析GLUT1基因rs841853位点基因型,采用递增负荷跑台运动试验获取运动员的生理机能指标,并检索追溯运动员截止到2017年各主项的个人最好成绩。结果:中国女子专业长跑运动员rs841853 G/T多态位点的基因型GG、GT和TT的频率分布为65.4%、29.5%和5.1%,等位基因G和T的频率分布为80.1%和19.9%,符合哈温平衡定律。基因型频率和等位基因频率与北京汉族人群存在显著差异(P<0.05)。GT+TT基因型携带者的6)VO2AT/6)VO2max、6)VE和5 000 m项目个人最好成绩显著高于GG基因型携带者(P<0.05)。结论:GLUT1基因rs841853位点的T等位基因可能是评估优秀长跑运动能力的基因标记,有待于通过扩大样本量和功能学研究给予进一步验证。

Objective：This study aimed at exploring the feasibility of glucose transporter 1（GLUT1） gene rs841853 polymorphism as a marker for evaluating and screening athletic ability of long distance runners.Methods：Seventy-eight Chinese female long distance runners participated in this research during 2009-2010; the genotypes of GLUT1 rs841853 were analyzed by matrix-assisted laser desorption ionization-time of flight mass spectrometry,and physiology indicators were determined by graded exercise testing on treadmill; personal best until2017 in main events were retrieved.Results：1） The genotype frequencies of rs841853 of Chinese female long distance runners were 65.4%,29.5% and 5.1% for GG,GT and TT,respectively,and allele frequencies were80.1% and 19.9% for G and T,respectively,these distributions were in Hardy-Weinberg equilibrium.These genotype frequencies and allele frequencies were significantly different from those of Han population in Beijing（Hap Map-HCB）（P 〈0.05）.GT and TT genotypes carriers showed greater 6）VO2 AT/6）VO2 maxand 6）VE,better personal best in 5 000 m running than GG genotype carriers（P〈 0.05）.Conclusion：The T allele of GLUT1 rs841853 could be a feasible genetic marker to evaluate and identify excellent long distance running performance,which needs to be further verified by further research with large sample size and function.