雌二醇和孕酮对小鼠子宫半胱亚磺酸脱羧酶基因表达和牛磺酸含量的调控

Regulatory effects of estradiol and progesterone on cysteine sulfinate decarboxylase gene expression and taurine content in the mouse uterus

实验主要研究了17β-雌二醇(E2)和孕酮(P4)对小鼠子宫组织中半胱亚磺酸脱羧酶基因(CSD)表达和牛磺酸含量的调控。给去卵巢小鼠分别皮下注射植物油(对照组)、17β-雌二醇(E2)、孕酮(P4)、E2和P4、E2和氟维司群(ICI 182780)、P4和米非司酮(RU486),24h后观察各组小鼠子宫中CSD的表达和牛磺酸的含量。Real-time PCR和Western blot定量分析结果显示,每只小鼠注射1μg E2能够显著抑制CSD的mRNA和蛋白的表达,6mg P4能够显著促进CSD mRNA和蛋白的表达,其受体抑制剂RU486和ICI182780都能逆转它们的作用。高效液相色谱(HPLC)测定结果显示,注射1μg E2和6mg P4分别下调和上调子宫组织中牛磺酸的含量。上述结果提示E2和P4可能通过受体途径参与调节子宫CSD的表达,从而调节子宫中牛磺酸含量。

The regulatory effects of estradiol （E2） and progesterone （P4） on expression of cysteine sulfinate decarboxylase gene （CSD） and the taurine content were studied in mouse uterus. Ovariectomized mice were injected with oil （control）, E2, P4, E2 and P4, E2 and fulvestrant （ICI182780）, P4 and mifepristone （RU486） respectively. The expression of CSD and the content of taurine in uterus were determined 24 h after injection. Quantitative real-time RT-PCR and Western blot results showed that the CSD mRNA and protein levels significantly decreased by treating with 1μg E2 and increased by treating with 6 mg P4. The effects of E2 and P4 can be abrogated by ICI182780 and RU486. The results of high performance liquid chromatography （HPLC） showed that the taurine content in the uterus was reduced by treating with 1μg E2 and increased by treating with 6 mg P4. These results demonstrated that the expression of CSD and the taurine content in response to E2 and P4 may be mediated by their receptors.