基于第三代测序技术的基因组组装方法及其在烟草中的应用

Genome assembly based on the third-generation sequencing technology and its application in tobacco

第三代测序技术凭借着片段读长更长的优势在基因组研究中得到广泛应用。为此,回顾了测序技术的发展,总结了三代测序技术的优缺点,重点对第三代测序技术的基因组组装方法,包括数据预处理、序列组装、组装之后的序列修补和序列的染色体定位方法进行了追踪和统计,同时介绍了测序技术和基因组组装方法在烟草基因组研究中的应用。

The third-generation sequencing technology has been widely used in genome research due to its advantage of long-fragment reads. The development of the sequencing technology was reviewed and the advantages and disadvantages of three different generations of sequencing technologies were summarized. Based on the third-generation sequencing, the methods of genome assembly including data pre-processing, sequence assembly, sequence repairing after assembly and chromosome localization methods were tracked and statistically analyzed. Application of sequencing technology and genome assembly in tobacco genome research was introduced.