阿尔茨海默病肾虚证病证结合动物模型的制备及验证

Preparation and validation of Alzheimer's disease with kidney deficiency syndrome animal model

目的:制备阿尔茨海默病肾虚证病证结合动物模型,并从行为学、组织形态学、生化指标进行验证,为阿尔茨海默病的中西医动物实验研究提供新的造模方法。方法:通过大鼠双侧海马注射Aβ25-35制造疾病模型,腹腔注射D-半乳糖制造亚急性衰老模拟中医肾虚证。从行为学、病理组织学判断疾病模型;抗氧化能力和HPA轴检验肾虚证模型;补肾名方地黄饮子干预后的各项指标的变化,反向验证病证结合动物模型。结果:造模后大鼠的学习记忆能力显著下降,海马部位出现了神经细胞凋亡,与假手术组比较差异具有统计学意义(P<0.05),说明疾病模型造模成功;模型组大鼠抗氧化能力下降,HPA轴功能虚性亢进(P<0.05),说明衰老已经造成肾虚证证候模型;地黄饮子干预后,以上指标均有一定程度的改善(P<0.05)。结论:阿尔茨海默病肾虚证病证结合动物模型制备成功。

Objective： To establish an animal model of Alzheimer＇s disease with ‘kidney deficiency＇ syndrome, and to verify it from behavioral, histomorphological and biochemical indicators, so as to provide a new modeling method for animal experiments of Alzheimer＇s disease. Methods： Aβ25-35 was injected into the rat hippocampus to make the disease model, and D-galactose was injected intraperitoneally to make subacute senescence of kidney deficiency syndrome. The disease model was judged from behaviourology and histopathology; kidney deficiency syndrome model was tested from antioxidant capacity and HPA axis; according to the changes in various indicators after the intervention of Dihuang Yinzi to verify the model reversely. Results： After modeling, the learning and memory ability of rats decreased significantly, and the neuronal apoptosis in hippocampus appeared. Compared with sham operation group, there was a statistical significance（P〈0.05）. It indicated that the disease model was successful. The antioxidation ability of the model group was decreased and the function of HPA axis was hyperfunction（P〈0.05）, indicating that aging has caused the syndrome model of kidney deficiency. After the intervention of Dihuang Yinzi, the above indexes were improved to some extent and were statistically significant（P〈0.05）. Conclusion： It proved that the modelling of animal models of Alzheimer＇s disease with ‘kidney deficiency＇ syndrome was successful.