长链非编码RNA XLOC-007123在乙型肝炎相关肝细胞癌中的表达及临床意义

Expression of the long non-coding RNA XLOC-007123 in hepatitis B-related hepatocellular carcinoma and its clinical significance

目的检测长链非编码RNA(lncRNA)XLOC-007123在乙型肝炎相关肝细胞癌(HCC)患者组织(癌、癌旁)及肝癌细胞系中的表达,并分析其表达水平与临床病理特征的关系。方法选取2013年12月-2015年12月于解放军三〇二医院肝胆外科行HCC切除术的52例乙型肝炎相关HCC患者的肝癌组织;同时取同一患者的癌旁组织作为对照。应用荧光实时定量PCR检测lncRNA XLOC-007123在乙型肝炎相关HCC患者癌组织、配对癌旁组织以及肝癌细胞系中的表达水平。其中研究所用细胞系L02、HuH7、HepG2、SMMC-7721均由解放军三○二医院临床管理中心提供。非参数Wilcoxon秩和检验比较lncRNA XLOC-007123在癌组织和配对癌旁组织的表达水平;独立样本t检验比较不同HCC细胞系与正常肝细胞系间lncRNA XLOC-007123表达水平。临床指标差异分析采用χ~2检验。结果与癌旁组织相比,lncRNA XLOC-007123在乙型肝炎相关HCC患者的癌组织中表达显著下调(P<0.01);与正常肝细胞株相比,IncRNA XLOC-007123在3种肝癌细胞系中均呈低表达(P值均<0.05)。lncRNA XLOC-007123表达水平与患者的血清AFP水平和肿瘤分期相关,差异有统计意义(χ2值分别为7.738、7.589,P值均<0.05)。结论 lncRNA XLOC-007123在乙型肝炎相关HCC组织中表达降低,且与临床患者血清AFP水平和病理分期有关联,推测lncRNA XLOC-007123在乙型肝炎相关HCC发生发展过程中发挥着调控作用,有望成为乙型肝炎相关HCC临床诊断的潜在标志物。

Objective To investigate the expression of the long non-coding RNA（lncRNA） XLOC-007123 in cancer and adjacent tissue,as well as hepatoma cell lines,in patients with hepatitis B-related hepatocellular carcinoma（HCC）,and the association between its expression level and clinicopathological features.Methods A total of 52 patients with hepatitis B-related HCC who underwent resection of HCC in Center of Hepatobiliary Surgery,302 Hospital of PLA,from December 2013 to December 2015 were enrolled,and HCC tissue samples were collected.Adjacent tissue samples collected from the same patient were used as controls.Quantitative real-time PCR was used to measure the expression of the lncRNA XLOC-007123 in HCC tissue,adjacent tissue,and hepatoma cell lines in the patients with hepatitis B-related HCC.Normal liver cell line LO2 and hepatoma cell lines HuH7,HepG2,and SMMC-7721 were obtained from Research Center for Clinical and Translational Medicine,302 Hospital of PLA.The nonparametric Wilcoxon rank sum test was used to compare the expression of the lncRNA XLOC-007123 between HCC tissue and adjacent tissue,and the independent samples t-test was used to compare the expression of the lncRNA XLOC-007123 between HCC cell lines and the normal liver cell line.The chi-square test was used for comparison of clinical indices.Results In the patients with hepatitis B-related HCC,the expression of the lncRNA XLOC-007123 was significantly lower in HCC tissue than in adjacent tissue（P〈0.01）; also,the expression of the lncRNA XLOC-007123 was significantly lower in HuH7,HepG2,and SMMC-7721 cells than in LO2 cells（all P〈0.05）.The expression of the lncRNA XLOC-007123 was associated with the serum level of alpha-fetoprotein（AFP） and tumor stage（χ2= 7.738 and 7.589,both P〈0.05）.Conclusion There is a reduction in the expression of the lncRNA XLOC-007123 in hepatitis B-related HCC tissue,which is associated with the serum level of AFP and pathological staging,suggesting that the lncRNA XLOC-007123 may exert a regulatory effect in the development and progression of hepatitis B-related HCC and may become a potential marker for the diagnosis of hepatitis B-related HCC.