Ⅱ型登革病毒前膜抗体对该病毒在THP-1细胞中复制能力的影响

Effect of presynaptic membrane antibody on replication capacity of dengue virus Ⅱ in THP-1 cells

目的探讨Ⅱ型登革病毒(dengue virusⅡ,DENVⅡ)前膜(presynaptic membrane,prM)抗体对DENVⅡ在THP-1细胞中复制能力的影响。方法采用不同稀释度(1/4~1/16 384)的DENVⅡanti-pr M单克隆抗体分别与不同MOI的DENVⅡ(MOI分别为3、0.75及0.19)复合感染THP-1细胞。建立DENVⅡ荧光定量PCR检测标准曲线,检测THP-1细胞培养上清液的病毒拷贝数。结果 DENVⅡ按MOI=3感染THP-1细胞,当prM稀释度为1/16和1/16 384时诱发THP-1细胞产生病毒的载量较高,prM稀释度为1/64和1/256时抑制病毒生长;DENVⅡ按MOI=0.75感染THP-1细胞,当prM稀释度1/16时可诱发更高浓度病毒载量;DENVⅡ按MOI=0.19感染THP-1细胞时,不会诱导产生高浓度的病毒载量。结论中浓度pr M抗体可抑制DENVⅡ在THP-1细胞中的复制能力,而高及低浓度prM抗体可促进DENVⅡ在THP-1细胞中的复制能力。

Objective To investigate the effect of presynaptic membrane （prM） antibody on replication capacity of dengue virus Ⅱ （DENV Ⅱ ） in THP-1 cells. Methods THP-1 cells were co-infected with anti-prM monoclonal antibody of DENV- Ⅱ, at various dilutions （1/4 - 1/16 384） and DENV Ⅱ at various MOIs （3, 0. 75 and 0. 19）. The standard curve of real-time fluorescent quantitative PCR for DENV Ⅱ was plotted and used for determination of virus copy number in culture supernatant of THP-1 cells. Results High virus load was induced in THP-1 cells infected with DENV Ⅱ at a MOI of 3 and prM dilutions of 1 / 16 and 1 / 16 384, while the virus growth was inhibited when the dilutions of prM were 1 / 64 and 1 / 256. Higher virus load was induced in the cells infected with DENV Ⅱ at a MOI of 0. 75 and a prM dilution of 1 / 16. However, the DENV II at a MOI of 0. 19 induced no high virus load. Conclusion The prM antibody at a moderate concentration inhibited the replication capacity of DENV Ⅱ in THP-1 cells, while those at high and low concentrations nromoted the renlication canacitv.