风干肠中产生物胺细菌的筛选鉴定及其产胺特性

Separation and Identification of Biogenic Amines Productive Strains from Dry Sausage and Their Biogenic Amine Formation Characteristics

以猪肉风干肠为研究对象,筛选鉴定产生物胺的微生物,并研究其产生物胺特性,以期对风干肠生物胺的控制技术提供试验依据。采用双层选择培养基显色法筛选产生物胺的菌落,并通过氨基酸脱羧酶试验确认产胺菌,采用全自动微生物生化鉴定仪进行菌种鉴定,并用高效液相色谱法分析不同菌种产生物胺的种类和能力。研究中筛选出两株产胺菌分别为大肠杆菌和克氏耶尔森菌,其中大肠杆菌主要产色胺、苯乙胺、腐胺、尸胺、酪胺,总产胺量为123.11μg/m L,尸胺产量最高为69.35μg/m L;克氏耶尔森菌主要产腐胺、酪胺,总产胺量为36.70μg/m L,酪胺产量最高为34.44μg/m L。

In order to reduce the content of biogenic amines of dry sausage, we isolated biogenic amines productive strains from dry sausage and studied their biogenic amine formation characteristics. Bacteria strains were screened by twolayer selection medium, and we used amino acid decarboxylase testto confirm the amine producing characteristics of the selected stains. Then the two biogenic amines productive strains were identified by automatic microorganism biochemistry instrument. And high performance liquid chromatography（HPLC） methodwas used to examine biogenic amine species and contents produced by each bacteria species. The identified strains were Escherichia coli and Yersinia kristensenii. Escherichia coli could produce tryptamine, phenylethylamine, putrescine, cadaverineand tyramine, the total biogenic amine content was 123.11 μg/m L, cadaverine showed the heighted production（69.35 μg/m L）. Yersinia kristensenii mainly produced tyramine, histamine, spermine, the total content of biogenic amines produced was 36.70 μg/m L, tyramine was34.44 μg/m L.