神经肽与针刺效应相关的研究

A Research of Correlation on Neuropeptide and Electro-acupuncture effect

本文用免疫组织化学方法和“蓝点”针感标记法,研究了神经肽(Sp,Vip、Npy)与针刺效应的关系。观察了肽能神经在针感(效)点和小肠中的分布规律。研究了针刺效应过程中脊髓背角神经肽的量变化。结果如下:1.针感(效)可出现于自皮肤到骨膜的不同层次;肽能神经、血管的出现率和针感(效)的出现率完全一致。2.三种肽能神经及其终末支呈串珠状,常伴随着血管,见于皮肤,皮下结缔组织,骨骼肌,小肠的肌层、粘膜下层,肠腺周围和绒毛的髓部。3.电针后,大鼠痛阈明显提高,其脊髓背角的神经肽含量,针刺侧明显地多于非针刺侧。

This article reported that the relation between neuropeptides(Sp. Vip.Npy) and electro-acupuncture effect had been studied with immunohistochemical method and 'blue point' label method of needling sensation. The rule of distribution of neuropeptides at the points and in the small intestines had been observed, and the quantitative changes of neuropeptides in the posterior gray column of spinal cord in the process of electro-acupuncture had been studied. These results showed that ①needling sensation could occur at different layers from the skin to the periosteum; the occurrence of needling sensation was the same as the peptidergic nerves and blood vessels; ②that the three kinds of peptidergic nerves and their terminals appeared string beads accompanying with blood vessels were seen in the skin, connective tissues of subcutaneous, skeletal muscles, small intestinal laminae muscularis, laminae submucosae, intestinal glands and cords of intestinal villi; ③after electro-needling given, the pain throshold in the rats was obviously higher than before. The neuropeptide content in the posterior gray column of spinal cord on the punctured side was greater than that of the non-punctured side.