不同基因型HCV感染患者血清Lp(a)水平检测分析

Analysis of Serum Lp(a)Levels in Patients with Different Genotypes of HCV Infection

目的探讨丙型肝炎病毒感染患者不同基因型与血清脂蛋白(a)水平的相关性。方法收集2016年6月~2017年6月在西安交通大学医学院第二附属医院就诊的105例HCV感染患者(男性59例,女性46例,平均年龄43.4±19.8岁)血清及同期健康对照患者30例血清标本和临床相关诊断资料,采用PCR-反向点杂交法检测HCV基因型分型,采用贝克曼AU5800全自动生化分析仪对其血清Lp(a)水平进行检测,两样本均数的比较采用t检验,多样本均数比较采用单因素方差分析。结果在105例HCV感染患者中共检出5种亚型,其中1b基因型31例,2a基因型30例,3a基因型19例,3b基因型12例,6a基因型13例;HCV感染组的血清Lp(a)水平(10.87±6.21mg/L)低于对照组(21.51±12.99mg/L),差异有统计学意义(t=6.281,P<0.000 1);3b,6a,2a,3a和1b五个基因型HCV感染患者的Lp(a)水平分别为12.51±6.11,9.75±5.73,12.28±7.63,9.22±4.47和10.37±5.75mg/L,均低于健康对照组,差异有统计学意义(t=2.288~4.355,均P<0.05);各个基因型之间Lp(a)水平差异无统计学意义(F=1.091,P=0.365 4)。结论与健康对照组相比,HCV感染能降低Lp(a)血清水平,且其在血清中表达水平与丙型肝炎的基因型不相关。

Objective To investigate whether serum Lp（a）level was related to the different genotypes of hepatitis C virus.Methods The serum and corresponding clinical data of 105 patients（male：59,female：46;average age：43.4±19.8）infected HCV and 30 healthy controls in the Second Affiliated Hospital of Xi＇an Jiaotong University from June 2016 to June 2017 were collected.HCV genotypes was detected by PCR-reverse point hybridization,all Lp（a）levels of serum were detected by using automatic biochemical analyzer AU5800 of Beckman.The comparison of the mean of the two samples was applied with t-test,analysis of variance was applied to compare among samples.Results There were 5 genotypes found in 105 patients,including 31 patients with genotype 1 bof HCV infection,30 patients with genotype 2 ainfection,19 patients with genotype3 ainfection,12 patients with genotype 3 binfection,13 patients with genotype 6 ainfection.The serum levels of Lp（a）in patients infected with HCV（10.87±6.21 mg/L）were significantly lower than that of healthy controls（21.51±12.99 mg/L）,the difference was statistically significant（t=6.281,P〈0.000 1）,meanwhile,the level of each genotype（3 b,6 a,2 a,3 a and 1 bwas 12.51±6.11,9.75±5.73,12.28±7.63,9.22±4.47和10.37±5.75 mg/L,respectively）was lower than that of healthy controls,respectively（t=4.355,3.356,3.965,2.288 and 3.119,all P〈0.05）,and the difference among genotypes was no statistically significant（F=1.091,P=0.365 4）.Conclusion Compared with healthy controls,HCV could reduce the expression of serum Lp（a）,but the serum Lp（a）levels were not associated with the genotypes of HCV.