重症急性胰腺炎大鼠舌组织小窝蛋白表达及大黄素干预的实验研究

Experimental study of expression of Caveolin-1 in tongue tissues of rats with severe acute pancreatitis and intervention of Emodin

目的探讨重症急性胰腺炎(SAP)大鼠舌组织小窝蛋白(Caveolin-1,Cav-1)表达及大黄素的调节作用。方法采用5%的牛磺胆酸钠逆行胆胰管注入制作大鼠重症急性胰腺炎模型。将SD雄性大鼠随机分为假手术组(SO组)、SAP模型组(SAP组)、大黄素治疗组(emodin组)。emodin组于造模术后即予大黄素(10 mg/kg,每日2次)灌胃;SAP组及SO组予等量生理盐水灌胃。造模5天后各组选取8只大鼠处死,留取标本,采用透射电镜进行组织形态学分析,Western blot、实时定量PCR等检测Cav-1蛋白及mRNA表达水平。结果透射电镜结果显示,与SO组比较,SAP组舌组织血管内皮细胞吞饮小泡明显增多;emodin组较SAP组明显减少。与SO组比较,SAP组舌组织中Cav-1蛋白及mRNA表达均上调,其中蛋白表达水平差异有统计学意义(P<0.05);emodin组较SAP组Cav-1蛋白及mRNA表达明显下调,差异有统计学意义(P<0.05)。结论 SAP大鼠舌组织Cav-1表达升高,大黄素干预可明显下调Cav-1的蛋白及mRNA表达。

Objective To investigate the expression of Caveolin-1（ Cav-1） in the tongue tissues of rats with severe acute pancreatitis and the regulating action of Emodin on it. Methods Severe acute pancreatitis model was induced by retrograde injection of 5% sodium taurocholate into the biliopancreatic duct. Male SD rats were randomly divided into model group of sham-operated group（ SO）,severe acute pancreatitis group（ SAP）,and Emodin-treated group（ Emodin）. After model establishment,Emodin was administrated orally in the Emodin group（ 10 mg/kg/each time,twice a day）,while an equal amount of normal saline was administrated in the SAP and SO group. 5 days after model establishment,analysis of histomorphology and expression of Cav-1 protein and mRNA were studied by Transmission Electron Microscope,Western-blot and Q-PCR using tongue tissues,which were collected from 8 sacrificed rats randomly chosen from each group. Results It was shown by Transmission Electron Microscope that pinocytosis bullules of vascular endothelial cell were more increased in the SAP group compared with the SO group,and fewer in the Emodin group. The expressions of Cav-1 and mRNA in the SAP group were increased compared with that of the SO group and the difference of expression was statistically significant（ P〈0. 05）. The expressions of Cav-1 and mRNA were significantly decreased in the Emodin group compared with the SAP group,and the difference of expression was statistically significant（ P〈0. 05）. Conclusion The expression of Cav-1 increases in SAP rats＇ tongue tissues; and the intervention of Emodin can down-regulate the expression of Cav-1 and mRNA.