摘要
聚丙烯腈是富腈基的高分子聚合物,易修饰改性,广泛应用于膜分离应用.我们以聚丙烯腈中空膜为载体,采用化学法交联聚乙烯亚胺并固定脂肪酶,固定过程中引入海藻酸钠,用CaCl_2进行后处理,得到固定化脂肪酶PAN-PEI-SA/E-CaCl_2载酶量为31.70(mg enzyme)/(g support),酶活为50.20 U/(g support),15次重复使用可保留58.77%的酶活,与游离酶相比耐酸性和耐温性有所提高,相同条件下与Nov 435相比,酶活更高,这表明最终得到的固定化脂肪酶有良好的工业应用前景.
Polyacrylonitrile is a nitrile-rich polymer and easy to be surface modified, and it has been widely applied in membrane separation. In this study, polyacrylonitrile hollow membrane was used as a support of enzyme immobilization by chemically cross-linking polyethyleneimine. Sodium alginate was added during immobilization, and the immobilized lipase was post-treated by CaCl2. The amount of loading enzyme of PAN-PEI-SA/E-CaCl2 was 31.70 (mg enzyme)/(g support) and the activity as 50.20 U/(g support).After 15 cycles of reactions, the immobilized enzyme retained 58.77% of initial enzyme activity, and showed an improvement of heat and acid resistance compared to free lipase. When it is compared to commercialized Nov 435 under same condition, PAN-PEI-SA/E-CaCl2 has higher enzyme activity during 15 cycles of reactions. It shows the immobilized lipase with PAN after surface modification has industrial application prospects.
出处
《分子催化》
EI
CAS
CSCD
北大核心
2018年第1期79-89,共11页
Journal of Molecular Catalysis(China)
基金
国家自然科学基金青年基金项目(No.31401674)
"十三五"国家重点研发计划项目(No.2016YFD0401400)~~
关键词
聚丙烯腈
表面改性
酶固定化
脂肪酶
polyacrylonitrile
surface modification
enzyme immobilization
lipase