TLR7激活上调细胞周期蛋白表达促进Hela细胞增殖

Activation of TLR7 up-regulation expression level of cyclins and promotes Hela cell proliferation

目的探讨Toll样受体7(TLR7)信号通路激活对人宫颈癌Hela细胞周期蛋白表达及对细胞增殖的影响。方法使用不同浓度的Gardiquimod经过不同的时间刺激Hela细胞,采用MTS比色法分析其对Hela细胞增殖的影响;采用流式细胞仪检测同一浓度的TLR7激动剂Gardiquimod经过不同的时间刺激Hela细胞后对Hela细胞周期的影响;Western blot分析Gardiquimod经过不同的时间刺激Hela细胞后对细胞周期蛋白Cyclin B1、Cyclin E表达水平的影响。结果 TLR7的激活促进Hela细胞增殖,并呈时间和浓度依赖性;Gardiquimod可时间依赖性增加细胞周期在S期的比例;Cyclin B1、Cyclin E的表达水平随着Gardiquimod作用时间的增加而增加。结论 TLR7激动剂Gardiquimod可能通过上调细胞周期蛋白Cyclin B1、Cyclin E表达水平,进而促进Hela细胞增殖。

Objective To explore the effects of TLR7 agonist-Gardiquimod on the proliferation and cell cycle of ac- tivation of TLR7 in Hela cells. Methods The cells were treated with different concentration of Gardiquimod for different times. MTS was performed to detect the impact of Gardiquimod on the proliferation of Hela ceils. Cell cycle were detected by flow cytometry ananlysis after treatment with different times of Gardiquimod and Western blot was used to analyze the expression of Cyelin B1 and Cyclin E. Results The activation of TLR7 could facilitate the proliferation of Hela cells, and existing dose and time dependence. Flow cytometry indicated that activation of TLR7 could arrest the cell cycle in S phase and Western Hot demonstrated that treatment with Gardiquimod could increase expression of Cyclin B1 and Cyclin E in a time dependence manner. Conclusion The activation of TLR7 may promote proliferation of Hela cells via increasing the expression of Cyclin B1 and Cyclin E.