二甲双胍丁酸盐对人肺腺癌A549细胞增殖、凋亡与迁移能力的影响

Effects of metformin butyrate on the proliferation,apoptosis and mobility of the A549 of the human lung adenocarcinoma

目的研究不同浓度的二甲双胍丁酸盐(MFB)作用不同时间后对人肺腺癌细胞A549增殖、凋亡与迁移能力的影响。方法用梯度浓度(0、0.5、1、2、4、8 mmol/L)的MFB对离体培养的A549细胞进行处理,在作用24、48 h后,用MTT法检测MFB对A549细胞增殖的抑制率;干预24、48 h后用流式细胞术分析A549细胞的凋亡率。用划痕实验分析MFB对A549细胞株迁移能力的影响。结果 MTT实验表明,MFB对A549细胞的增殖具有抑制作用,并且在一定浓度范围内呈现出浓度和时间依赖性(P<0.05)。流式细胞术检测结果显示:实验组细胞凋亡率大于对照组(P<0.05),具有一定的时间和浓度依赖性(P<0.05)。划痕实验结果显示实验组细胞迁移率小于对照组,高浓度用药组细胞迁移率小于低浓度用药组(P<0.05),且药物作用48 h后细胞迁移率<24 h(P<0.05)。结论 MFB在体外能够抑制人肺腺癌A549细胞的增殖和迁移,促进其凋亡,其强度可能与其浓度和作用时间有关。

Objective To study the effects of different concentrations of metforminbutyrate （MFB） on the prolifera- tion, apoptosis and mobility of the human lung adenocarcinoma （A549） after different time of intervention. Methods A549 cells cultered in vitro were treated with MFB at a gradient concentration （0, 0.5, 1, 2, 4, 8 mmol/ L）, and after 24 h and 48 h, the MFB＇s inhibition rate on the A549 cell proliferation was detected by MTr. The apoptosis rate of A549 cells was analyzed by using a fluid cell instrument after 24 h and 48 h. The effect of MFB on the mobility of A549 cell lines was analyzed with scratch test. Results The MFB experiment showeded that MFB had a inhibitory effect on the proliferation of 549 cells with a dose and time dependent manner（ P 〈 0.05 ）. The experimental results showed that the apoptosis rate of the experimental group was greater than that of the control group （ P 〈 0. 05 ）, which had a certain time and concentration dependence （P 〈 0. 05 ）. The experimental results of the scratches showed that the experimental group cell migration rate was less than the control group, high concentration treatment group cell migration rate was less than the low concentration treatment group （P 〈 0. 05 ）, and drug action after 48 h cell migration rate was less than 24 h （ P 〈 0. 05 ）. Conclusion MFB can inhibit the proliferation and migration of human pulmonary adenocarcinoma A549 cells in vitro and promote apoptosis, and its intensity may be related to its concentration and time.