子宫内膜息肉干细胞的提取及内皮抑素对其血管生成的影响

Isolation of endometrial polyps stem cells and the effect of endostatin on the angiogenesis of endometrial polyps

目的探讨子宫内膜息肉(EP)组织中血管内皮生长因子(VEGF)的表达水平和重组人血管内皮抑素对EP血管生成作用的影响。方法免疫组化法观察EP组织与正常内膜组织中VEGF的表达。胶原酶进行体外分离培养子宫内膜息肉干细胞(EPMSCs),运用RT-PCR和流式细胞术检测干细胞相关标志物,并进行多向分化能力鉴定。ELISA法检测EPMSCs与正常子宫内膜干细胞(EMSCs)VEGF的表达。ELISA法检测内皮抑素对EMSCs VEGF表达的影响。结果EP组织中VEGF表达明显增强(P<0.001)。成功分离出EPMSCs和EMSCs,VEGF的表达前者较后者明显增多(P<0.001)。内皮抑素能够使EPMSCs分泌的VEGF显著降低(P<0.01)。结论 VEGF与EP的发生有关。EP可能成为成体干细胞的组织来源。内皮抑素能够通过抑制血管生成发挥治疗EP的作用。

Objective To explore the expression of vascular endothelial growthfactor（VEGF） in endometrial polyps and the effect of endostatin on the angiogenesis of endometrial polyps. Methods Immunohistochemistry was used to detect the expression levels of endometrial polyps tissues and normal endometrial tissues. By using collagenase, EPMSCs were isolated and cultured. By using RT-PCR and flow cytometry, the expression of stem cells related markers was detected. The capacity of EPMSCs and EMSCs to differentiate in vitro was evaluated. The VEGF secreted by EPMSCs and EMSCs was detected by ELISA. The effect of endostatin on VEGF of EPMSCs was evaluated. Results The expression of VEGF increased significantly （ P 〈 0. 001 ）. EPMSCs and EMSCs were isolated successfully and the expression level of VEGF of EPMSCs was signifiantly higher than that of EMSCs（P 〈0. 001 ）. Endostatin could supress the expression of VEGF secreted by EPMSCs（P 〈0. 01）. Conclusion VEGF is related to the occurrence of endometrial polyps. SP tissues may become a source of adult stem cells sources. Endostatin can play a role in the treatment of EP by inhibiting angiogenesis