益气活血解毒方对小鼠卵巢癌ID-8细胞株增殖及血管生成过程的影响

Effect of Yiqi Huoxue Jiedu Decoction on Proliferation of Ovarian Cancer ID-8 Cells and Angiogenesis

目的：研究益气活血解毒方抑制鼠卵巢上皮癌ID-8细胞株增殖的作用,并观察本方对于脐静脉内皮细胞（HUVECs）形成血管过程的作用,为本方发挥疗效提供实验依据。方法：采用细胞增殖与检测方法（cell counting kit-8,CCK-8）检测本方对于ID-8细胞增殖的抑制作用;将含中药血清、磁珠分选的调节性T细胞（regulatory T cells,Tregs）与抗血管内皮生长因子（VEGF）抗体分组共培养,观察血管生成情况差异。结果：与空白组比较,10%,20%含药血清均能够抑制卵巢上皮癌ID-8细胞株的增殖,此抑制作用在培养96 h时最明显;在72,96 h时,20%含药血清组的抑制率明显高于10%含药血清组（P〈0.05,P〈0.01）。与Tregs（-）各组相比,Tregs（＋）各组HUVECs细胞呈现明显的血管网状结构,中药含药血清组血管成网不明显,VEGF抗体联合含药血清组血管成网情况最差。结论：益气活血解毒方对小鼠卵巢上皮癌ID-8细胞株的增殖过程有显著的抑制作用,同时能够干预血管的形成,这也许与本方影响Tregs促进肿瘤新生血管的作用相关。

Objective： To analyze the inhibitory effects of Yiqi Huoxue Jiedu decoction on proliferation of ovarian epithelial carcinoma ID-8 cells in vitro,observe the effect of this method on angiogenesis of human umbilical vein endothelial cells（HUVECs）,and provide experimental basis for its efficacy.Method： Cell counting kit-8（CCK-8） was used to testify the inhibitory effect of Yiqi Huoxue Jiedu decoction on the proliferation of ovarian cancer ID-8 cells; decoction serum,regulatory T cells（Tregs） isolated by immune magnetic MACS Micro Beads,as well as vascular endothelial growth factors（VEGF） were co-cultured,and then the angiogenesis of HUVEC was observed.Result： As compared with blank control group（fetal bovine serum group）,drug serum groups with concentration of 10% and 20% showed inhibitory effects on ID-8 cells,and this effect was most significant at 96 h.Serum group with concentration of 20% showed remarkable advantages against 10% group at72 h（P 〈0.01） and 96 h（P〈0.05）.HUVECs cultivated with Treg cells exhibited a better angiogenesis process.On the other hand,the vascular network was not obvious in drug serum group,and the inhibitory effect was most significant in anti-VEGF ＋ Yiqi Huoxue Jiedu decoction.Conclusion： Yiqi Huoxue Jiedu decoction can restrict the multiplication of ovarian epithelial carcinoma ID-8 cells,and it could intervene the angiogenesis of HUVECs,which is probably associated with the intervention on Tregs.