摘要
目的应用同位素相对标记与绝对定量技术(iTRAQ)分析β-地中海贫血患者血浆蛋白,筛选出差异蛋白及其功能分析。方法通过强阳离子交换色谱分析法的8种不同同位素标记的i TRAQ和液相色谱法以及串联质谱分析了地贫组和健康照者血浆蛋白。通过Mascot软件分析和GO分析表达差异的蛋白。结果与对照组相比,实验组中共鉴定出有定量信息的蛋白质58个,包括49个上调蛋白和9个下调蛋白。生物信息学分析显示差异表达的蛋白主要参与凝血、凝固止血、伤口愈合、应对受伤、体液调节的水平、急性炎症反应、囊泡、介导转运、血小板激活、血小板脱粒等过程,这些表达差异的蛋白中选择5个显著表达差异作为候选蛋白,分别是CRP、SAA1、HBA、FBLN1、HRG。结论本研究发现这5种蛋白在β-地中海贫血发病过程中发挥重要作用,可能作为该病潜在的临床筛查生物标志物。
Objective：Using isobaric tags for relative and absolute quantification（iTRAQ）technology to analyze the proteins in serum of β-thalassaemias and find the differentially expressed proteome. Methods：Analyzing the total proteins in serum samples from patients with β-thalassaemias and samples of healthy controls by using eight-plex iTRAQ added with strong cation exchange choematography and liquid chromatography coupled with tandem mass spectrometry. The differentially expressed proteins were identified by Mascot and GO. Results：A total of 58 differentially expressed proteins were identified,including 49 proteins were up-regulation and 9 proteins were down-regulation. Bioinformatics analysis revealed that differential expression proteins were mainly related to the blood coagulation Coagulation,hemostasis,wound healing,response to wounding,regulation of body fluid levels,acute inflammatory response,vesicle-mediated transport,platelet activation,and platelet degranulation process. Among of them,there were 5 proteins significant differentially expressed,including CRP,SAA1,HBA,FBLN1,and HRG. Conclusion：This study found that 5 kinds of protein in children with mycoplasma pneumonia play an important role in the process,promisingly as the disease clinical screening of potential biomarkers.
出处
《中国优生与遗传杂志》
2018年第2期9-12,共4页
Chinese Journal of Birth Health & Heredity
基金
深圳市卫生计生系统项目(201506008)
深圳市科技计划项目(JCYJ20150403101146298)
