高氟在孕期和哺乳期对仔鼠脑发育及单胺类神经递质的影响

Effects of high fluoride on rat brain development and monoamine neurotransmitters during pregnancy and lactation

目的探讨高氟在孕期和哺乳期对仔鼠脑发育及单胺类神经递质的影响。方法 24只Wistar雌性大鼠,交配成功后,随机分为对照组(蒸馏水),低剂量染氟组(5mg/kg),高剂量染氟组(20mg/kg),各8只孕鼠,自妊娠开始染毒至仔鼠生后3周断乳(孕期和哺乳期染氟),断乳后给予染氟喂养至仔鼠生后6周神经系统发育完全,每组保留20只仔鼠。分别测量仔鼠生后1周、3周、6周的体重;仔鼠6周时Morris水迷宫定位航行测试;ELISA法检测海马组织中多巴胺(DA)、5-羟色胺(5-HT)含量。采用硫代巴比妥酸法检测脑组织匀浆中丙二醛(MDA)含量,采用黄嘌呤氧化酶法检测脑组织匀浆中超氧化物歧化酶(SOD)含量。结果第1周,第3周,仔鼠体重增加逐渐增加,各组间无明显差异。第6周,高剂量染氟组体重明显低于对照组,差异有统计学意义P<0.05。与对照组比较,高剂量染氟组第1天、第2天、第3天、第4天定位航行潜伏期均明显延长,差异无统计学意义P>0.05。与对照组比较,高剂量染氟组DA、5-HT均明显降低,差异有统计学意义P<0.05。与对照组比较,高剂量染氟组、低剂量染氟组的MDA均明显增高、SOD均明显降低,差异有统计学意义P<0.05。与低剂量染氟组比较,高剂量染氟组的MDA均明显增高、SOD均明显降低,差异有统计学意义P<0.05。结论高氟影响孕期和哺乳期仔鼠的学习记忆能力,与降低单胺类神经递质含量有关。

Objective：To investigate the effects of high fluoride on the development of rat brain and monoamine neurotransmitters during pregnancy and lactation. Methods：24 Wistar female rats,after the success of the mating,（distilled water）were randomly divided into control group,low-dose fluoride group（5 mg/kg）,high dose fluoride group（20 mg/kg）,the only 8 pregnant mouse,since pregnancy might be to young rats born three weeks after weaning（during pregnancy and lactation with fluorine）,after ablactation to dye fluorine to lactation born after 6 weeks of nervous system development,completely retained 20 young mice in each group. 1 week,3 weeks and 6 weeks weight of the rat were measured separately. In the 6 weeks of the mouse,the Morris water maze locates the navigation test. ELISA was used to detect the content of dopamine（DA）and serotonin（5-ht）in the hippocampus. The content of malondialdehyde（MDA）in brain tissue homogenation was detected by thiobarbital acid method,and the content of SOD was detected by xanthine oxidase method. Results：In week 1,week 3,the weight gain increased gradually and there was no significant difference between the groups. In week 6,the weight of the high-dose fluorine group was significantly lower than the control group,and the difference was statistically significant P0.05. Compared with the control group,the incubation period of the high-dose fluorine group was significantly prolonged on day 1,2,3 and 4,and the difference was not statistically significant P BBB 0. 0.05. Compared with the control group,the DA and 5-ht were significantly reduced in the high-dose fluorine group,and the difference was statistically significant P0.05. Compared with the control group,the MDA of high-dose FCS and low-dose fluorine groups significantly increased and SOD decreased significantly,and the difference was statistically significant P0.05. Compared with low-dose fluorine group,the MDA of high-dose FCS was significantly higher and SOD was significantly lower,and the difference was statistically significant P0.05. Conclusion：High fluoride affects the learning and memory ability of pregnant and lactating mice,which is related to reducing the content of monoamine neurotransmitter.