糖尿康胶囊通过AMPK/CREB信号通路治疗2型糖尿病作用研究

Study on the Effect of Tangniaokang Capsule on Type 2 Diabetes Mellitus via AMPK/CREB Signaling Pathway

目的探讨糖尿康胶囊对2型糖尿病大鼠的治疗作用机制。方法以腹腔注射小剂量STZ并辅以高脂饮食喂养建立2型糖尿病大鼠模型,观察不同剂量的糖尿康胶囊对糖尿病大鼠空腹血糖(FBG)、血清胰岛素(FINS)、甘油三酯(TG)、血清总胆固醇(TC)和游离脂肪酸(FFA)水平的影响。采用相应试剂盒测定LDH和Caspase3水平。Westernbloting检测蛋白磷酸化水平。结果模型组糖尿病大鼠的FBG、TG、TC和FFA较对照组明显升高,糖尿康胶囊治疗4周后,各项指标均有所降低,与模型组相比有显著性差异。糖尿康胶囊治疗可以增加糖尿病大鼠血清胰岛素水平,且显著降低糖尿病大鼠体内LDH和Caspase3水平,减少胰岛损伤。Westernbloting结果发现其可以促进AMPK和CREB蛋白磷酸化。结论糖尿康胶囊能改善2型糖尿病的胰岛素抵抗状态,减轻胰岛损伤,其作用机制可能通过激活AMPK/CREB信号通路。

Objective： To investigate the mechanism of Tangniaokang capsule in treating type 2 diabetic rats. Method： Rat models of type 2 diabetes were established by small dose of intraperitoneal injection of STZ combined with high fat diet,the effect of different doses of Tangniaokang capsule on the rats＇ fasting blood glucose（ FBG）,serum insulin（ FINS）,triglyceride（ TG）,serum total cholesterol（ TC） and free fatty acid（ FFA） levels were observed. The levels of LDH and Caspase3 were measured with the corresponding kits. Westernbloting was used to detect the level of phosphorylation of protein. Result： FBG,TG,TC and FFA of diabetic rats in model group were significantly higher than those in the control group. After 4 weeks of treatment by Tangniaokang capsule,all indicators were decreased,compared with themodel group,there was a significant difference. Diabetic capsule can increase the level of serum insulin and significantly reduce the level of LDH and Caspase3 in diabetic rats,and reduce pancreatic islet injury. Westernbloting results showed that it could promote the phosphorylation of AMPK and CREB proteins. Conclusion： The diabetic capsule can improve the insulin resistance of type 2 diabetes and reduce islet damage and the mechanism of its action may be activated by the activation of the AMPK/CREB signaling pathway.