墨旱莲石油醚提取物对STZ糖尿病大鼠生化指标及肾组织病理学的影响

Effect of petroleum ether extract of Eclipta prostrate on biochemical parameters and histopathology of kidney in STZ induced diabetic rats

目的探究墨旱莲石油醚提取物对链脲佐菌素(streptozotocin,STZ)诱导的糖尿病大鼠血清中血糖、胰岛素、甘油三酯、胆固醇、肌酐、尿素、尿酸等生化指标以及肾组织病理学的影响。方法采用高能饲料喂养8周加腹腔注射STZ(30 mg·kg^(-1))的方法建立2型糖尿病大鼠模型。造模成功后,随机分为模型对照组、二甲双胍阳性对照组、实验组,分别连续经口灌胃给予蒸馏水、二甲双胍(400 mg·kg^(-1))、墨旱莲石油醚提取物不同剂量(100、200、400 mg·kg^(-1))4周。利用相应试剂盒检测大鼠血清中血糖、胰岛素、甘油三酯、胆固醇、肌酐、尿素、尿酸的含量;利用光镜观察肾脏组织病理学变化;采用Western blot检测肾脏组织中TNF-α蛋白的表达。结果与模型组相比,墨旱莲石油醚提取物各剂量均能降低血糖、甘油三酯、胆固醇、肌酐、尿素、尿酸含量,升高胰岛素含量(P<0.01或P<0.05),且呈现剂量依赖关系,同时有效减轻肾脏组织的病理损伤,下调肾脏TNF-α蛋白的表达。结论墨旱莲石油醚提取物能有效改善STZ诱导糖尿病大鼠的相关生化指标,并对肾脏具有一定的保护作用,该研究为墨旱莲的进一步开发利用提供了理论依据。

Aim To investigate the effect of petroleum ether extract of Eclipta prostrata（ PEEEP） on biochemical parameters such as blood glucose,insulin,total glycerides,total cholesterol,creatinine,urea nitrogen,uric acid levels and histopathology of kidney in STZ induced diabetic rats. Methods The model of type 2 diabetic rats was established by feeding with high energy diet for 8 weeks and injecting streptozotocin（ 30 mg·kg^（-1））,after making the model successfully,the rats were randomly divided into model control group,metformin positive control group and experimental group, and gavaged with distilled water, metformin（ 400 mg·kg^（-1）） and different dose of PEEEP（ 100,200,400 mg · kg^（-1）） for 4 weeks,respectively. The contents of blood glucose,insulin,total glycerides,total cholesterol,creatinine,urea nitrogen,uric acid were detected by corresponding kit,the pathological changes of kidney were observed by light microscopy,and the expression of TNF-α protein was detected by Western blot. Results Compared with model control group,PEEEP could reduce blood glucose,total glycerides,total cholesterol,creatinine,urea nitrogen,uric acid and increase the content of insulin in a dosedependent manner（ P〈 0. 01 or P 〈0. 05）. It also could alleviate the pathological damage of kidney tissues and down-regulate the expression of TNF-α in kidney tissues. Conclusions PEEEP could effectively improve related biochemical parameters in streptozotocin induced diabetic rats,and it has protective effect on the kidney,providing the theoretical basis for further exploitation and utilization of Eclipta prostrata.