半夏多糖脱蛋白工艺研究

Study on Deproteinization of Polysaccharides from Pinellia Ternate

目的：筛选半夏多糖除蛋白的最优工艺。方法：以半夏多糖保留率和蛋白脱除率为考察指标,研究3种脱蛋白方法对半夏多糖脱蛋白的影响,并用正交试验优选出最佳工艺。结果：确定了胰蛋白酶结合三氯乙酸的脱蛋白方法,最优脱蛋白工艺为：在胰蛋白酶最适酶解条件下酶解,酶解液与TCA溶液（5%）的体积比为4：1,振摇时间10 min,静置时间30 min;在此条件下半夏多糖蛋白脱除率为94.96%,多糖保留率为91.51%。结论：优选出的脱蛋白方法可以有效的去除蛋白,纯化多糖,为半夏多糖制备工艺的进一步研究提供了参考。

Objective： To screen the optimum method for deproteinization of Pinellia Ternate polysaccharides. Methods： Three deproteinization methods were evaluated in terms of the ratio protein removing and polysaccharide remaining by using orthogonal test to select the best technology. Results： Trypsin-trichloroacetic acid method was the best one,in which deproteination rate was94. 96% and 91. 51% of polysaccharide was remained. The optimum conditions for deproteinization were as follows. Under the best condition of the enzyme trypsin hydrolysis,the volume ratio of the enzyme solution to TCA solution was 4： 1,the shaking time was 10 min,and the settling time was 30 min. Conclusion： The optimal method of deproteination was better,which provided a reference for the further study of the preparation technology of Pinellia Ternate polysaccharides.