TGFβ-SMAD信号通路基因在梅山猪与杜洛克猪不同级别卵泡中的表达分析

Expression Patterns of Genes of TGFβ-SMAD Signaling Pathway in Meishan and Duroc Follicle of Different Sizes

为探索TGFβ-SMAD信号通路基因在卵泡中的表达规律,采用Real-time PCR方法检测TGFβ-SMAD信号通路基因THBS1、DCN、LTBP1、TGFβ1、TGFβ2、TGFβ3、TGFβRⅠ、TGFβRⅡ、SMAD2、SMAD3、SMAD4基因在梅山、杜洛克S、M1、M2、L卵泡中的表达量变化。结果表明:TGFβ配体1、2、3型及正向调控因子THBS1、LTBP1、负向调控因子DCN、受体TGFβRⅠ、TGFβRⅡ以及受体调控SMAD2、SMAD3和辅SMAD4都在梅山与杜洛克的中等卵泡中存在表达差异,TGFβ1基因、TGFβRⅠ基因在梅山猪各级卵泡中的表达量均极显著高于杜洛克(P<0.01)。研究提示,TGFβ-SMAD基因可能参与卵泡发育过程。

In order to explore the expression patterns of TGFβ-SMADsignaling pathway genes in ovarian follicle,ovarian follicle were collected from 3Meishan and 3Duroc sows and classified by diameter.The relative expression amount of TGF beta-SMAD pathway genes(THBS1,DCN,LTBP1,TGF beta 1,beta 2,beta 3,TGFβRⅠ,TGFβRⅡ,SMAD2,SMAD3,SMAD4)in S,M1,M2,and L ovarian follicle of Meishan and Duroc sows were detected by Real-time PCR method.The results showed that:TGF beta1 beta 2,beta 3,THBS1,LTBP1,DCN,TGFβRⅠ,TGFβRⅡand SMAD2,SMAD3,SMAD4 were differentially expressed in secondary follicles of Meishan and Duroc.Especially the expression of TGFβ1and TGFβRⅠin S,M1,M2 and L follicle of Meishan were significantly higher than that in Duroc.The results suggested that TGFβ-SMAD pathway may be involved in the process of follicular development in pig.