一株副猪嗜血杆菌的初步分离鉴定及药敏试验

Isolation,Identification and Drug Sensitivity Test of a Haemophilus Parasuis Strain

为了解重庆渝东地区副猪嗜血杆菌耐药谱,筛选获得敏感抗菌素,对来自重庆云阳县某规模猪场3头送检病仔猪关节液从细菌显微形态特点、培养特性、生化试验及动物回归试验进行疑似副猪嗜血杆菌的分离鉴定;纸片法对分离菌株进行药敏试验。结果表明,从3头送检病仔猪关节液初步分离鉴定出一株副猪嗜血杆菌;该分离菌株对头孢类药品极度敏感;对青霉素类及喹诺酮类药品耐药,这和该地区规模猪场用药习惯、种猪的来源地密切相关。

In order to know the extent of Haemophilus parasuis resistance to drugs in the east Chongqing and to screen sensitive antibiotic for Haemophluis parasuis,a suspected Haemophilus parasuis is isolated from the hock fluid of three piglets from a scale pig farm in Yuyang county by microscopic morphological characteristics,cultural characteristics,biochemical tests and animal regression test;then disk diffusion method is adopted to conduct drug sensitivity test for the isolated bacterial strain.The results show that a suspected Haemophilus parasuis strain had been isolated from three diseased piglets;the isolated Haemophilus parasuis strain is strongly sensitive to Cephalosporinantibiotics,and resistant to Penicillinantibiotics and Quinolone antibiotics,which is closely related to the habit of using drug and the source of piglets in east Chongqing.