杠柳苷元大鼠在体肠吸收动力学研究

Studies on Absorption Kinetics of Periplogenin in Rat Intestine

目的：考察杠柳苷元大鼠在体肠吸收动力学,探讨杠柳苷元可能的吸收机制。方法：采用大鼠在体单向肠灌流模型,使用高效液相色谱法以Hypersil ODS2（4.6 mm×250 mm,5μm）色谱柱为固定相,乙腈-水（29∶71）为流动相,流速1 mL·min^（-1）,于220 nm波长测定灌流液中杠柳苷元的含量。结果：杠柳苷元不同浓度下的吸收速率常数（Ka）和表观吸收系数（Papp）在考察浓度范围内（1.25、5、20μg·mL^（-1））具有显著性差异（P〈0.01）,存在浓度依赖性。结论：杠柳苷元的肠吸收机制除被动扩散外,可能还涉及主动转运。

Objective：To clarify the intestinal absorption characteristics of periplogenin in rats.Methods：With the in situ rat single-pass intestinal perfusion model,the perfusate samples were protein precipitated with methanol.After sample preparation,the samples were separated on an Elite Hypersil ODS2（4.6 mm×250 mm,5 μm）column and detected at 220 nm using acetonitrile and water（29∶71）as mobile phase.HPLC method was used to determine the concentrations of periplogenin in perfusion fluid.Results：The absorption rat constant（Ka）and apparent absorption coefficient（Papp）of periplogenin showed significant difference（P0.01）at different concentrations（1.25,5,20 μg·mL^（-1））in a concentrationdependent manner.Conclusion：In addition to the passive diffusion,the active transport may be one of mechanisms of the intestinal absorption of periplogenin.